Sunitinib induces primary ectopic endometrial cell apoptosis through up-regulation of STAT1 in vitro.

ABSTRACT:

Background

Endometriosis (EMS) is a prevalent gynecological condition characterized by the growth of endometrial tissue outside the uterine cavity. This study aimed to clarify the targeted therapeutic effect of sunitinib in an endometriosis in vitro experiment.

Methods

Primary culture of ectopic endometrial cells and normal endometrial cells. Six tumor targeting drugs were selected to screen. MTT was used to determine the IC50, flow cytometry, and DAPI staining of the targeted drugs, in order to determine the apoptosis. The differential proteins after seeding were analyzed by protein spectrum, the correlation between the specific protein and cell apoptosis was determined by small molecule interference, and the expression of each related protein was detected by Western blot.

SUBMITTER: Li J 

PROVIDER: S-EPMC7676178 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature