Structural basis of superinfection exclusion by bacteriophage T4 Spackle.
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ABSTRACT:
SUBMITTER: Shi K
PROVIDER: S-EPMC7677548 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Publications
Structural basis of superinfection exclusion by bacteriophage T4 Spackle.
Shi Ke K Oakland Justin T JT Kurniawan Fredy F Moeller Nicholas H NH Banerjee Surajit S Aihara Hideki H
Communications biology 20201119 1
A bacterial cell infected with T4 phage rapidly establishes resistance against further infections by the same or closely related T-even-type bacteriophages - a phenomenon called superinfection exclusion. Here we show that one of the T4 early gene products and a periplasmic protein, Spackle, forms a stoichiometric complex with the lysozyme domain of T4 tail spike protein gp5 and potently inhibits its activity. Crystal structure of the Spackle-gp5 lysozyme complex shows that Spackle binds to a hor ...[more]
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