Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology.

ABSTRACT: This work demonstrates a novel high-throughput (HT) microfluidics-enabled uninterrupted perfusion system (HT-?UPS) and validates its use with chronic all-optical electrophysiology in human excitable cells. HT-?UPS consists of a soft multichannel microfluidic plate cover which could button on a commercial HT 96-well plate. Herein, we demonstrate the manufacturing process of the system and its usages in acute and chronic all-optical electrophysiological studies of human induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CM) and engineered excitable (spiking HEK) cells. HT-?UPS perfusion maintained functional voltage and calcium responses in iPSC-CM and spiking HEK cells under spontaneous conditions and under optogenetic pacing. Long-term culture with HT-?UPS improved cell viability and optogenetically-tracked calcium responses in spiking HEK cells. The simplicity of this design and its compatibility with HT all-optical electrophysiology can empower cell-based assays for personalized medicine using patient-derived cells.

SUBMITTER: Wei L 

PROVIDER: S-EPMC7680692 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature