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Oscillating lncRNA Platr4 regulates NLRP3 inflammasome to ameliorate nonalcoholic steatohepatitis in mice.


ABSTRACT: Background: Understanding the molecular events and mechanisms underlying development and progression of nonalcoholic steatohepatitis is essential in an attempt to formulating a specific treatment. Here, we uncover Platr4 as an oscillating and NF-?B driven lncRNA that is critical to the pathological conditions in experimental steatohepatitis Methods: RNA-sequencing of liver samples was used to identify differentially expressed lncRNAs. RNA levels were analyzed by qPCR and FISH assays. Proteins were detected by immunoblotting and ELISA. Luciferase reporter, ChIP-sequencing and ChIP assays were used to investigate transcriptional gene regulation. Protein interactions were evaluated by Co-IP experiments. The protein-RNA interactions were studied using FISH, RNA pull-down and RNA immunoprecipitation analyses Results: Cyclic expression of Platr4 is generated by the core clock component Rev-erb? via two RevRE elements (i.e., -1354/-1345 and -462/-453 bp). NF-?B transcriptionally drives Platr4 through direct binding to two ?B sites (i.e., -1066/-1056 and -526/-516 bp), potentially accounting for up-regulation of Platr4 in experimental steatohepatitis. Intriguingly, Platr4 serves as a circadian repressor of Nlrp3 inflammasome pathway by inhibiting NF-?B-dependent transcription of the inflammasome components Nlrp3 and Asc. Loss of Platr4 down-regulates Nlrp3 inflammasome activity in the liver, blunts its diurnal rhythm, and sensitizes mice to experimental steatohepatitis, whereas overexpression of Platr4 ameliorates the pathological conditions in an Nlrp3-dependent manner. Mechanistically, Platr4 prevents binding of the NF-?B/Rxr? complex to the ?B sites via a physical interaction, thereby inhibiting the transactivation of Nlrp3 and Asc by NF-?B. Conclusions: Platr4 functions to inactivate Nlrp3 inflammasome via intercepting NF-?B signaling. This lncRNA might be an attractive target that can be modulated to ameliorate the pathological conditions of steatohepatitis.

SUBMITTER: Lin Y 

PROVIDER: S-EPMC7681083 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Oscillating lncRNA <i>Platr4</i> regulates <i>NLRP3</i> inflammasome to ameliorate nonalcoholic steatohepatitis in mice.

Lin Yanke Y   Wang Shuai S   Gao Lu L   Zhou Ziyue Z   Yang Zemin Z   Lin Jingpan J   Ren Shujing S   Xing Huijie H   Wu Baojian B  

Theranostics 20210101 1


<b>Background:</b> Understanding the molecular events and mechanisms underlying development and progression of nonalcoholic steatohepatitis is essential in an attempt to formulating a specific treatment. Here, we uncover Platr4 as an oscillating and NF-κB driven lncRNA that is critical to the pathological conditions in experimental steatohepatitis <b>Methods:</b> RNA-sequencing of liver samples was used to identify differentially expressed lncRNAs. RNA levels were analyzed by qPCR and FISH assay  ...[more]

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