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Astaxanthin accumulation in the green microalga Haematococcus pluvialis: Effect of initial phosphate concentration and stepwise/continuous light stress.

ABSTRACT: Nutrient composition and light stress significantly affect the productivity of astaxanthin in Haemotococcus pluvialis. Hence, the present study aimed to investigate the effect of initial phosphate concentration and two distinct light regimes on astaxanthin accumulation in H. pluvialis. In the green stage, microalgae were cultivated in different initial phosphate concentrations under 2000?lx and a 12:12?h photoperiod. To initiate astaxanthin accumulation, an increased light intensity of 5000?lx was provided using two methods; (i) stepwise light stress, where a 12:12?h photoperiod was provided for 14 days, followed by 14 days of continuous illumination, and (ii) continuous illumination for 28 days. Phosphate limitation and continuous light stress were favourable to enhance cellular astaxanthin accumulation, which reached 7% by weight. The highest astaxanthin concentration of 27.0?±?1.9?mg/L and lowest specific light energy consumption of 32.9?±?2.3?kW?h/g astaxanthin were reported in cultures grown in 41?mg/L phosphate under continuous light stress.

SUBMITTER: Liyanaarachchi VC

PROVIDER: S-EPMC7683316 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Astaxanthin accumulation in the green microalga Haematococcus pluvialis: Effect of initial phosphate concentration and stepwise/continuous light stress.

Liyanaarachchi Vinoj Chamilka VC Nishshanka Gannoru Kankanamalage Sanuji Hasara GKSH Premaratne Rankoth Gedara Malith Malsha RGMM Ariyadasa Thilini Udayangani TU Nimarshana Pemaththu Hewa Viraj PHV Malik Anushree A

Biotechnology reports (Amsterdam, Netherlands) 20201008

Nutrient composition and light stress significantly affect the productivity of astaxanthin in Haemotococcus pluvialis. Hence, the present study aimed to investigate the effect of initial phosphate concentration and two distinct light regimes on astaxanthin accumulation in H. pluvialis. In the green stage, microalgae were cultivated in different initial phosphate concentrations under 2000 lx and a 12:12 h photoperiod. To initiate astaxanthin accumulation, an increased light intensit ...[more]

PMID: 33294401

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Project description:The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark-grown cells. Although moderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5?mg?L(-1) ?day(-1) ) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark-grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high-light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5?mg?L(-1) ?day(-1) by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark-grown cells under photo-induction conditions. Biotechnol. Bioeng. 2016;113: 2088-2099. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

Dataset Information

Astaxanthin accumulation in the green microalga Haematococcus pluvialis: Effect of initial phosphate concentration and stepwise/continuous light stress.

Publications

Astaxanthin accumulation in the green microalga <i>Haematococcus pluvialis</i>: Effect of initial phosphate concentration and stepwise/continuous light stress.

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