Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis.
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ABSTRACT:
SUBMITTER: Haas TM
PROVIDER: S-EPMC7684580 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Publications
Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis.
Haas Thomas M TM Qiu Danye D Häner Markus M Angebauer Larissa L Ripp Alexander A Singh Jyoti J Koch Hans-Georg HG Jessen-Trefzer Claudia C Jessen Henning J HJ
The Journal of organic chemistry 20200622 22
The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts. ...[more]
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