Periostin regulates autophagy through integrin ?5?1 or ?6?4 and an AKT-dependent pathway in colorectal cancer cell migration.

ABSTRACT: Colorectal cancer (CRC) is one of the most fatal cancers with highly invasive properties. The progression of CRC is determined by the driving force of periostin (PN) from cancer-associated fibroblasts (CAFs) in the tumour microenvironment. This present work aims to investigate autophagy-mediated CRC invasion via the receptor integrin (ITG) by PN. The level of PN in 410 clinical CRC tissues was found increased and was an independent poor prognosis marker (HR = 2.578, 95% CI = 1.218-5.457, P-value = .013) with a significant correlation with overall survival time (P-value < .001). PN activated proliferation, migration and invasion of CRC cells, but with reduced autophagy. Interestingly, the reduction of LC3 autophagic protein corresponded to the increased ability of CRC cell migration. The siITG?5-treated HT-29 and siITG?4-treated HCT-116 CRC cells attenuated epithelial-to-mesenchymal transitions (EMT)-related genes and pAKT compared with those in siITG-untreated cells. The reduction of pAKT by a PI3K inhibitor significantly restored autophagy in CRC cells. These evidences confirmed the effect of PN through either ITG?5?1 or ITG?6?4 and the AKT-dependent pathway to control autophagy-regulated cell migration. In conclusion, these results exhibited the impact of PN activation of ITG?5?1 or ITG?6?4 through pAKT in autophagy-mediated EMT and migration in CRC cells.

SUBMITTER: Thongchot S 

PROVIDER: S-EPMC7686974 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature