Dataset Information

Correlation between hemolytic profile and phylotype of Cutibacterium acnes (formerly Propionibacterium acnes) and orthopedic implant infection.

ABSTRACT:

Introduction

Cutibacterium acnes is a recognized culprit for implant-associated infections, but positive cultures do not always indicate clinically relevant infection. Studies have shown a correlation between the β-hemolytic phenotype of C. acnes and its infectious capacity, but correlation with genetic phylotype has not been performed in literature. The purpose of this study is to evaluate β-hemolysis phenotype, genetic phylotype, and mid-term clinical outcomes of C. acnes isolated from orthopedic surgical sites.

Methods

Fifty-four C. acnes isolates previously obtained from surgical wounds of patients undergoing hip, knee, shoulder, or spine implant removal were re-cultured. There were 21 females and 33 males with an average age of 59 years (range, 18-84). Twenty-four were from clinically infected sites whereas 30 were considered contaminants. De novo β-hemolysis was analyzed and a retrospective chart review was performed to evaluate clinical outcomes at 7.1 years (range, 0.1-12.8).

Results

On Brucella agar with 5% rabbit blood, 46% of contaminant and 43% of infectious isolates were hemolytic. Type II phylotype was significantly more nonhemolytic regardless of infectious or contaminant status (p < 0.05). Type 1B correlated with a hemolytic-infectious phenotype and Type 1A with a hemolytic-contaminant phenotype but was not statistically significant.

Conclusion

The β-hemolytic profile of C. acnes did not correlate with phylotype or clinically relevant orthopedic infection.

SUBMITTER: Lee J

PROVIDER: S-EPMC7689609 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

Correlation between hemolytic profile and phylotype of <i>Cutibacterium acnes</i> (formerly <i>Propionibacterium acnes</i>) and orthopedic implant infection.

Lee Julia J Greenwood Quaintance Kerryl E KE Schuetz Audrey N AN Shukla Dave R DR Cofield Robert H RH Sperling John W JW Patel Robin R Sanchez-Sotelo Joaquin J

Shoulder & elbow 20190809 6

<h4>Introduction</h4><i>Cutibacterium acnes</i> is a recognized culprit for implant-associated infections, but positive cultures do not always indicate clinically relevant infection. Studies have shown a correlation between the β-hemolytic phenotype of <i>C. acnes</i> and its infectious capacity, but correlation with genetic phylotype has not been performed in literature. The purpose of this study is to evaluate β-hemolysis phenotype, genetic phylotype, and mid-term clinical outcomes of <i>C. ac ...[more]

PMID: 33281943

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