Modular and Versatile Trans-Encoded Genetic Switches.
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ABSTRACT:
SUBMITTER: Paul A
PROVIDER: S-EPMC7689881 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Publications
Modular and Versatile Trans-Encoded Genetic Switches.
Paul Avishek A Warszawik Eliza M EM Loznik Mark M Boersma Arnold J AJ Herrmann Andreas A
Angewandte Chemie (International ed. in English) 20200727 46
Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans-encoded and based on tRNA-mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs, including RNA, small molecules, and proteins. This powerful approach enables control of the translation of protein expression from plasmid and genome DNA. ...[more]
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