Reagentless Amperometric Pyruvate Biosensor Based on a Prussian Blue- and Enzyme Nanoparticle-Modified Screen-Printed Carbon Electrode.

ABSTRACT: We report a facile strategy for developing reagentless amperometric pyruvate biosensors based on enzyme nanoparticles (EnNPs). The EnNPs were prepared using pyruvate oxidase crosslinked with graphene quantum dots. Before EnNP immobilization, screen-printed carbon electrodes (SPCEs) were modified with Prussian blue, a biocompatible coordination polymer. The biosensor system was optimized in terms of the working potential and pH value. At pH 7.0 in 50 mM phosphate-buffered solution, the biosensor showed optimal characteristics under an applied potential of -0.10 V versus an internal pseudo-Ag reference electrode. Using these optimized conditions, the biosensor performance was characterized via the chronoamperometric technique. The EnNP-immobilized SPCE exhibited a dynamic linear range from 10 to 100 ?M for pyruvate solution, and a sensitivity of 40.8 ?A mM^-1 cm^-2 was recorded. The observed detection limit of the biosensor was 0.91 ?M (S/N = 3) and it showed strong anti-inference capability under the optimized working potential. Furthermore, the practical applicability of the proposed biosensor was studied in fish serum samples.

SUBMITTER: Thirumalai D 

PROVIDER: S-EPMC7689939 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature