Dataset Information

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids.

ABSTRACT: It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy.

SUBMITTER: Attoye B

PROVIDER: S-EPMC7692145 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids.

Attoye Bukola B Pou Chantevy C Blair Ewen E Rinaldi Christopher C Thomson Fiona F Baker Matthew J MJ Corrigan Damion K DK

Biosensors 20201028 11

It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measur ...[more]

PMID: 33126531

Similar Datasets

Project description:This article presents the capabilities and selected measurement results from the newly developed low-cost air pollution measurement system mounted on an unmanned aerial vehicle (UAV). The system is designed and manufactured by the authors and is intended to facilitate, accelerate, and ensure the safety of operators when measuring air pollutants. It allows the creation of three-dimensional models and measurement visualizations, thanks to which it is possible to observe the location of leakage of substances and the direction of air pollution spread by various types of substances. Based on these models, it is possible to create area audits and strategies for the elimination of pollution sources. Thanks to the usage of a multi-socket microprocessor system, the combination of nine different air quality sensors can be installed in a very small device. The possibility of simultaneously measuring several different substances has been achieved at a very low cost for building the sensor unit: 70 EUR. The very small size of this device makes it easy and safe to mount it on a small drone (UAV). Because of this device, many harmful chemical compounds such as ammonia, hexane, benzene, carbon monoxide, and carbon dioxide, as well as flammable substances such as hydrogen and methane, can be detected. Additionally, a very important function is the ability to perform measurements of PM2.5 and PM10 suspended particulates. Thanks to the use of UAV, the measurement is carried out remotely by the operator, which allows us to avoid the direct exposure of humans to harmful factors. A big advantage is the quick measurement of large spaces, at different heights above the ground, in different weather conditions. Because of the three-dimensional positioning from GPS receiver, users can plot points and use colors reflecting a concentration of measured features to better visualize the air pollution. A human-friendly data output can be used to determine the mostly hazardous regions of the sampled area.

Dataset Information

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids.

Publications

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets