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Coupled Metabolic Cycles Allow Out-of-Equilibrium Autopoietic Vesicle Replication.

ABSTRACT: We report chemically fuelled out-of-equilibrium self-replicating vesicles based on surfactant formation. We studied the vesicles' autocatalytic formation using UPLC to determine monomer concentration and interferometric scattering microscopy at the nanoparticle level. Unlike related reports of chemically fuelled self-replicating micelles, our vesicular system was too stable to surfactant degradation to be maintained out of equilibrium. The introduction of a catalyst, which introduces a second catalytic cycle into the metabolic network, was used to close the first cycle. This shows how coupled catalytic cycles can create a metabolic network that allows the creation and perseverance of fuel-driven, out-of-equilibrium self-replicating vesicles.

SUBMITTER: Engwerda AHJ

PROVIDER: S-EPMC7692917 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Coupled Metabolic Cycles Allow Out-of-Equilibrium Autopoietic Vesicle Replication.

Engwerda Anthonius H J AHJ Southworth Josh J Lebedeva Maria A MA Scanes Robert J H RJH Kukura Philipp P Fletcher Stephen P SP

Angewandte Chemie (International ed. in English) 20200903 46

We report chemically fuelled out-of-equilibrium self-replicating vesicles based on surfactant formation. We studied the vesicles' autocatalytic formation using UPLC to determine monomer concentration and interferometric scattering microscopy at the nanoparticle level. Unlike related reports of chemically fuelled self-replicating micelles, our vesicular system was too stable to surfactant degradation to be maintained out of equilibrium. The introduction of a catalyst, which introduces a second ca ...[more]

PMID: 32706135

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