Tomato Oil Encapsulation by ?-, ?-, and ?-Cyclodextrins: A Comparative Study on the Formation of Supramolecular Structures, Antioxidant Activity, and Carotenoid Stability.

ABSTRACT: Cyclodextrins (CDs) are oligosaccharides, comprising 6 (?), 7 (?), or 8 (?) glucose residues, used to prepare oil-in-water emulsions and improve oil stability towards degradation. In this research, the aptitude of ?-, ?-, and ?-CDs to form complexes with a supercritical CO2 extracted lycopene-rich tomato oil (TO) was comparatively assessed. TO/CD emulsions and the resulting freeze-dried powders were characterized by microscopy, Fourier transform infrared-attenuated total reflection (FTIR-ATR), and differential scanning calorimetry (DSC), as well as for their antioxidant activity. Furthermore, carotenoid stability was monitored for 90 days at 25 and 4 °C. Confocal and SEM microscopy revealed morphological differences among samples. ?- and ?-CDs spontaneously associated into microcrystals assembling in thin spherical shells (cyclodextrinosomes, Ø ? 27 µm) at the oil/water interface. Much smaller (Ø ? 9 µm) aggregates were occasionally observed with ?-CDs, but most TO droplets appeared "naked". FTIR and DSC spectra indicated that most CDs did not participate in TO complex formation, nevertheless structurally different interfacial complexes were formed. The trolox equivalent antioxidant capacity (TEAC) activity of emulsions and powders highlighted better performances of ?- and ?-CDs as hydrophobic antioxidants-dispersing agents across aqueous media. Regardless of CDs type, low temperature slowed down carotenoid degradation in all samples, except all-[E]-lycopene, which does not appear efficiently protected by any CD type in the long storage period.

SUBMITTER: Durante M 

PROVIDER: S-EPMC7693019 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature