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Acid treatment of Atlantic salmon (Salmo salar) scales prior to analysis has negligible effects on ?13C and ?15N isotope ratios.


ABSTRACT: There is debate in the literature as to whether scales of fishes require acidification to remove inorganic carbonates prior to stable isotope analysis. Acid-treated and untreated scales from 208 Atlantic salmon from nine locations on both sides of the Atlantic were analysed for ?13C and ?15N. Linear mixed-effect models determined the effect of acid treatment to be statistically significant. However, the mean difference was small (?13C 0.1?±?0.2‰, ?15N -0.1?±?0.2‰) and not of biological relevance. This study concludes that Atlantic salmon scales do not need to be acidified prior to stable isotope analysis.

SUBMITTER: O'Toole C 

PROVIDER: S-EPMC7693048 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Acid treatment of Atlantic salmon (Salmo salar) scales prior to analysis has negligible effects on δ<sup>13</sup>C and δ<sup>15</sup>N isotope ratios.

O'Toole Christina C   Weigum Emily E   Graham Conor T CT   White Philip P   Samways Kurt K   Hayden Brian B   Brophy Deirdre D  

Journal of fish biology 20200909 4


There is debate in the literature as to whether scales of fishes require acidification to remove inorganic carbonates prior to stable isotope analysis. Acid-treated and untreated scales from 208 Atlantic salmon from nine locations on both sides of the Atlantic were analysed for δ<sup>13</sup>C and δ<sup>15</sup>N. Linear mixed-effect models determined the effect of acid treatment to be statistically significant. However, the mean difference was small (δ<sup>13</sup>C 0.1 ± 0.2‰, δ<sup>15</sup>N  ...[more]

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