Dataset Information

Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature.

ABSTRACT: Body temperature is an important physiological parameter in many studies of laboratory mice. Continuous assessment of body temperature has traditionally required surgical implantation of a telemeter, but this invasive procedure adversely impacts animal welfare. Near-infrared thermography provides a non-invasive alternative by continuously measuring the highest temperature on the outside of the body (T_skin), but the reliability of these recordings as a proxy for continuous core body temperature (T_core) measurements has not been assessed. Here, T_core (30 s resolution) and T_skin (1 s resolution) were continuously measured for three days in mice exposed to ad libitum and restricted feeding conditions. We subsequently developed an algorithm that optimised the reliability of a T_skin-derived estimate of T_core. This identified the average of the maximum T_skin per minute over a 30-min interval as the optimal way to estimate T_core. Subsequent validation analyses did however demonstrate that this T_skin-derived proxy did not provide a reliable estimate of the absolute T_core due to the high between-animal variability in the relationship between T_skin and T_core. Conversely, validation showed that T_skin-derived estimates of T_core reliably describe temporal patterns in physiologically-relevant T_core changes and provide an excellent measure to perform within-animal comparisons of relative changes in T_core.

SUBMITTER: van der Vinne V

PROVIDER: S-EPMC7693264 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature.

van der Vinne Vincent V Pothecary Carina A CA Wilcox Sian L SL McKillop Laura E LE Benson Lindsay A LA Kolpakova Jenya J Tam Shu K E SKE Krone Lukas B LB Fisk Angus S AS Wilson Tatiana S TS Yamagata Tomoko T Cantley James J Vyazovskiy Vladyslav V VV Peirson Stuart N SN

Scientific reports 20201126 1

Body temperature is an important physiological parameter in many studies of laboratory mice. Continuous assessment of body temperature has traditionally required surgical implantation of a telemeter, but this invasive procedure adversely impacts animal welfare. Near-infrared thermography provides a non-invasive alternative by continuously measuring the highest temperature on the outside of the body (T<sub>skin</sub>), but the reliability of these recordings as a proxy for continuous core body te ...[more]

PMID: 33244132

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Project description:BackgroundWhole body vibration exercise (WBVE) has been shown to improve muscular strength and power, and increase peripheral blood flow. The aim of this study was to evaluate the behavior of the skin temperature (Tsk) on regions of the lower limbs from an acute bout of WBVE.Methods and findingsCross-sectional study approved by local ethics committee (Certificado de Apresentação para Apreciação Ética-CAAE-19826413.8.0000.5259) and Trial registration (Registro Brasileiro de Ensaios Clínicos-REBEC-RBR-738wng). Using Infrared thermography (IRT), Tsk and thermal symmetry of the posterior lower extremities (thigh, knee, calf and heel) were examined in 19 healthy participants. IRT was assessed during 60-second WBVE exposures of 0, 30 and 50 Hz. From the adjusted linear mixed effects model, vibration frequency, time and regions of the lower extremity were significant (p<0.001). However, the variable laterality was not significant (p = 0.067) and was excluded from the adjusted statistical model. The adjusted model was significant (p<0.00001) and all variables in the model were significant (p<0.01) indicating that Tsk decreases with time, independently of the vibration frequency. The value of the Pseudo-R-Squared for the model was 0.8376. The presented mathematical model of the current study may be useful to justify the patterns observed for all vibration frequencies between and 0 and 50 Hz. The main limitations of the study were the reduced time of the intervention and not having evaluated other regions of the body.ConclusionsThe acute exposure of 60-second mechanical vibration has effect on the behavior of Tsk of the posterior region of the lower limbs, which is likely to be associated with a decrease on the blood flow due to WBVE. It is speculated that during WBVE a greater supply of blood is required where the body responds by shunting blood flow from the skin to working muscle in the first seconds of exercise. Further investigative work is required to verify this hypothesis.

Dataset Information

Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature.

Publications

Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature.

Similar Datasets

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