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Mitochondrial ROS prime the hyperglycemic shift from apoptosis to necroptosis.


ABSTRACT: We have previously identified a shift from TNF-?-induced apoptosis to necroptosis that occurs under hyperglycemic conditions. This shift involves the downregulation or silencing of caspases and concurrent upregulation of necroptotic proteins leading to activation of the necrosome. In addition, under hyperglycemic conditions in vivo, this shift in cell death mechanisms exacerbates neonatal hypoxia-ischemia (HI) brain injury. Here, we identify two major factors that drive the hyperglycemic shift to necroptosis: (1) reactive oxygen species (ROS) and (2) receptor-interacting protein kinase 1 (RIP1). ROS, including mitochondrial superoxide, led to the oxidation of RIP1, as well as formation and activation of the necrosome. Concurrently, ROS mediate a decrease in the levels and activation of executioner caspases-3, -6, and -7. Importantly, hyperglycemia and mitochondrial ROS result in the oxidation of RIP1 and loss of executioner caspases prior to death receptor engagement by TNF-?. Moreover, RIP1 partially controlled levels of mitochondrial ROS in the context of hyperglycemia. As a result of its regulation of ROS, RIP1 also regulated necrosome activation and caspase loss. Mitochondrial ROS exacerbated neonatal HI-brain injury in hyperglycemic mice, as a result of the shift from apoptosis to necroptosis.

SUBMITTER: Deragon MA 

PROVIDER: S-EPMC7693268 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Mitochondrial ROS prime the hyperglycemic shift from apoptosis to necroptosis.

Deragon Matthew A MA   McCaig William D WD   Patel Payal S PS   Haluska Robert J RJ   Hodges Alexa L AL   Sosunov Sergey A SA   Murphy Michael P MP   Ten Vadim S VS   LaRocca Timothy J TJ  

Cell death discovery 20201126 1


We have previously identified a shift from TNF-α-induced apoptosis to necroptosis that occurs under hyperglycemic conditions. This shift involves the downregulation or silencing of caspases and concurrent upregulation of necroptotic proteins leading to activation of the necrosome. In addition, under hyperglycemic conditions in vivo, this shift in cell death mechanisms exacerbates neonatal hypoxia-ischemia (HI) brain injury. Here, we identify two major factors that drive the hyperglycemic shift t  ...[more]

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