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Rapid de novo evolution of lysis genes in single-stranded RNA phages.

ABSTRACT: Leviviruses are bacteriophages with small single-stranded RNA genomes consisting of 3-4 genes, one of which (sgl) encodes a protein that induces the host to undergo autolysis and liberate progeny virions. Recent meta-transcriptomic studies have uncovered thousands of leviviral genomes, but most of these lack an annotated sgl, mainly due to the small size, lack of sequence similarity, and embedded nature of these genes. Here, we identify sgl genes in 244 leviviral genomes and functionally characterize them in Escherichia coli. We show that leviviruses readily evolve sgl genes and sometimes have more than one per genome. Moreover, these genes share little to no similarity with each other or to previously known sgl genes, thus representing a rich source for potential protein antibiotics.

SUBMITTER: Chamakura KR

PROVIDER: S-EPMC7693330 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Rapid de novo evolution of lysis genes in single-stranded RNA phages.

Chamakura Karthik R KR Tran Jennifer S JS O'Leary Chandler C Lisciandro Hannah G HG Antillon Sophia F SF Garza Kameron D KD Tran Elizabeth E Min Lorna L Young Ry R

Nature communications 20201126 1

Leviviruses are bacteriophages with small single-stranded RNA genomes consisting of 3-4 genes, one of which (sgl) encodes a protein that induces the host to undergo autolysis and liberate progeny virions. Recent meta-transcriptomic studies have uncovered thousands of leviviral genomes, but most of these lack an annotated sgl, mainly due to the small size, lack of sequence similarity, and embedded nature of these genes. Here, we identify sgl genes in 244 leviviral genomes and functionally charact ...[more]

PMID: 33243984

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