Unknown

Dataset Information

0

Equine Penile Squamous Cell Carcinomas as a Model for Human Disease: A Preliminary Investigation on Tumor Immune Microenvironment.


ABSTRACT: Penile squamous cell carcinomas (SCCs) are common tumors in older horses, with poor prognosis mostly due to local invasion and recurrence. These tumors are thought to be mainly caused by Equus caballus papillomavirus type 2 (EcPV-2). The aim of this study is to characterize the tumor immune environment (TIME) in equine penile tumors. Equine penile epithelial tumors (17 epSCCs; 2 carcinomas in situ, CIS; 1 papilloma, P) were retrospectively selected; immune infiltrate was assessed by histology and immunohistochemistry; RT-qPCR tested the expression of selected chemokines and EcPV-2 DNA and RNA. The results confirmed EcPV-2-L1 DNA in 18/20 (90%) samples. L1 expression was instead retrieved in 13/20 cases (65%). The samples showed an increased infiltration of CD3+lymphocytes, macrophages (MAC387; IBA1), plasma cells (MUM1), and FoxP3+lymphocytes in the intra/peritumoral stroma when compared to extratumoral tissues (p < 0.05). Only MAC387+neutrophils were increased in EcPV-2high viral load samples (p < 0.05). IL12/p35 was differentially expressed in EcPVhigh and EcPVlow groups (p = 0.007). A significant decrease of IFNG and IL2 expression was highlighted in TGFB1-positive samples (p < 0.05). IBA1 and CD20 were intratumorally increased in cases where IL-10 was expressed (p < 0.005). EpSCCs may represent a good spontaneous model for the human counterpart. Further prospective studies are needed in order to confirm these preliminary results.

SUBMITTER: Porcellato I 

PROVIDER: S-EPMC7693514 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Equine Penile Squamous Cell Carcinomas as a Model for Human Disease: A Preliminary Investigation on Tumor Immune Microenvironment.

Porcellato Ilaria I   Mecocci Samanta S   Mechelli Luca L   Cappelli Katia K   Brachelente Chiara C   Pepe Marco M   Orlandi Margherita M   Gialletti Rodolfo R   Passeri Benedetta B   Ferrari Angelo A   Modesto Paola P   Ghelardi Alessandro A   Razzuoli Elisabetta E  

Cells 20201027 11


Penile squamous cell carcinomas (SCCs) are common tumors in older horses, with poor prognosis mostly due to local invasion and recurrence. These tumors are thought to be mainly caused by <i>Equus caballus</i> papillomavirus type 2 (EcPV-2). The aim of this study is to characterize the tumor immune environment (TIME) in equine penile tumors. Equine penile epithelial tumors (17 epSCCs; 2 carcinomas <i>in situ</i>, CIS; 1 papilloma, P) were retrospectively selected; immune infiltrate was assessed b  ...[more]

Similar Datasets

| S-EPMC8508821 | biostudies-literature
| S-EPMC8300259 | biostudies-literature
| S-EPMC6004546 | biostudies-literature
| S-EPMC9745380 | biostudies-literature
| S-EPMC7281394 | biostudies-literature
| S-EPMC8188514 | biostudies-literature
| S-EPMC6292793 | biostudies-literature
| S-EPMC7217868 | biostudies-literature
| S-EPMC7407624 | biostudies-literature
| S-EPMC7469863 | biostudies-literature