Dataset Information

Overexpression Levels of LbDREB6 Differentially Affect Growth, Drought, and Disease Tolerance in Poplar.

ABSTRACT: The application of drought stress-regulating transcription factors (TFs) offers a credible way to improve drought tolerance in plants. However, many drought resistant TFs always showed unintended adverse effects on plant growth or other traits. Few studies have been conducted in trees to evaluate and overcome the pleiotropic effects of drought tolerance TFs. Here, we report the dose-dependent effect of the Limonium bicolor LbDREB6 gene on its overexpression in Populus ussurensis. High- and moderate-level overexpression of LbDREB6 significantly increased drought tolerance in a dose-dependent manner. However, the OE18 plants showed stunted growth under normal conditions, but they were also more sensitive to Marssonina brunnea infection than wild type (WT) and OE14 plants. While, OE14 showed normal growth, the pathogen tolerance of them was not significantly different from WT. Many stress-responsive genes were up-regulated in OE18 and OE14 compared to WT, especially for OE18 plants. Meanwhile, more pathogen tolerance related genes were down-regulated in OE18 compared to OE14 and WT plants. We achieved improved drought tolerance by adjusting the increased levels of exogenous DREB genes to avoid the occurrence of growth reduction and reduced disease tolerance.

SUBMITTER: Yang J

PROVIDER: S-EPMC7693672 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Overexpression Levels of LbDREB6 Differentially Affect Growth, Drought, and Disease Tolerance in Poplar.

Yang Jingli J Wang Hanzeng H Zhao Shicheng S Liu Xiao X Zhang Xin X Wu Weilin W Li Chenghao C

Frontiers in plant science 20201111

The application of drought stress-regulating transcription factors (TFs) offers a credible way to improve drought tolerance in plants. However, many drought resistant TFs always showed unintended adverse effects on plant growth or other traits. Few studies have been conducted in trees to evaluate and overcome the pleiotropic effects of drought tolerance TFs. Here, we report the dose-dependent effect of the Limonium bicolor LbDREB6 gene on its overexpression in Populus ussurensis. H ...[more]

PMID: 33304356

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Project description:Background and aimsSoil salinization and aridification are swiftly engulfing the limited land resources on which humans depend, restricting agricultural production. Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is important in the biosynthesis of terpenoids, which are involved in plant growth, development and responses to environmental stresses. This study aimed to provide guidance for producing salt- and drought-resistant poplar.MethodsA protein expression system was used to obtain PtHMGR protein, and high-performance liquid chromatography was used to detect the activity of PtHMGR protein in vitro. In addition, a simplified version of the leaf infection method was used for transformation of 'Nanlin895' poplar (Populus×euramericana). qRT-PCR was used to identify expression levels of genes.Key resultsPtHMGR catalysed a reaction involving HMG-CoA and NADPH to form mevalonate. Overexpression of PtHMGR in Populus × euramericana 'Nanlin895' improved drought and salinity tolerance. In the presence of NaCl and PEG6000, the rates of rooting and survival of PtHMGR-overexpressing poplars were higher than those of wild-type poplars. The transgenic lines also exhibited higher proline content and peroxidase and superoxide dismutase activities, and a lower malondialdehyde level under osmotic stress. In addition, the expression of genes related to reactive oxygen species (ROS) scavenging and formation was altered by osmotic stress. Moreover, the effect of osmotic stress on transcript levels of stress-related genes differed between the transgenic and wild-type poplars.ConclusionPtHMGR catalysed a reaction involving HMG-CoA and NADPH to form mevalonate in vitro. Overexpression of PtHMGR promoted root development, increased the expression of ROS scavenging-related genes, decreased the expression of ROS formation-related genes, and increased the activity of antioxidant enzymes in transgenic poplars, enhancing their tolerance of osmotic stress. In addition, overexpression of PtHMGR increased expression of the stress-related genes KIN1, COR15 and AAO3 and decreased that of ABI, MYB, MYC2 and RD22, enhancing the stress resistance of poplar.

Project description:The drought-tolerant 'Ningchun 47' (NC47) and drought-sensitive 'Chinese Spring' (CS) wheat (Triticum aestivum L.) cultivars were treated with different PEG6000 concentrations at the three-leaf stage. An analysis on the physiological and proteomic changes of wheat seedling in response to drought stress was performed. In total, 146 differentially accumulated protein (DAP) spots were separated and recognised using two-dimensional gel electrophoresis. In total, 101 DAP spots representing 77 unique proteins were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. These proteins were allocated to 10 groups according to putative functions, which were mainly involved in carbon metabolism (23.4%), photosynthesis/respiration (22.1%) and stress/defence/detoxification (18.2%). Some drought stress-related proteins in NC47, such as enolase, 6-phosphogluconate dehydrogenase, Oxygen-evolving enhancer protein 2, fibrillin-like protein, 2-Cys peroxiredoxin BAS1 and 70-kDa heat shock protein, were more upregulated than those in CS. Multivariate principal components analysis revealed obvious differences between the control and treatments in both NC47 and CS, while cluster analysis showed that the DAPs displayed five and six accumulation patterns in NC47 and CS, respectively. Protein-protein interaction network analysis showed that some key DAPs, such as 2-Cys peroxiredoxin BAS1, RuBisCO large subunit-binding protein, 50S ribosomal protein L1, 6-phosphogluconate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase isoenzyme and 70-kDa heat shock protein, with upregulated accumulation in NC47, had complex interactions with other proteins related to amino acid metabolism, carbon metabolism, energy pathway, signal transduction, stress/defence/detoxification, protein folding and nucleotide metabolism. These proteins could play important roles in drought-stress tolerance and contribute to the relatively stronger drought tolerance of NC47.

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Overexpression Levels of LbDREB6 Differentially Affect Growth, Drought, and Disease Tolerance in Poplar.

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Overexpression Levels of <i>LbDREB6</i> Differentially Affect Growth, Drought, and Disease Tolerance in Poplar.

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