Dataset Information

Comparison of Pesticide Residue Levels in Red Wines from Canary Islands, Iberian Peninsula, and Cape Verde.

ABSTRACT: In this work, the QuEChERS method coupled to liquid chromatography-time-of flight-mass spectrometry and gas chromatography-triple quadrupole-mass spectrometry were applied for the evaluation of pesticide residues and risk assessment in red wines. The methodologies were successfully validated for 173 pesticides. Recovery values were in the range 75-100% for almost all pesticides and limits of quantification were between 2.60 and 21.39 µg/kg, which are in good agreement with the maximum residue limits (MRLs) established by the European Commission for pesticides in wine grapes. Finally, the analysis of 84 red wine samples from the Canary Islands, the Iberian Peninsula, and Cape Verde was carried out, which found the presence of 31 pesticide residues. However, the risk assessment disclosed that despite the large number of pesticides and the concentrations found, which in some cases exceeded the MRLs, the consumption of these wines, without considering a possible cumulative effect, does not entail a risk to the consumers.

SUBMITTER: Santana-Mayor A

PROVIDER: S-EPMC7694142 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Comparison of Pesticide Residue Levels in Red Wines from Canary Islands, Iberian Peninsula, and Cape Verde.

Santana-Mayor Álvaro Á Rodríguez-Ramos Ruth R Socas-Rodríguez Bárbara B Díaz-Romero Carlos C Rodríguez-Delgado Miguel Ángel MÁ

Foods (Basel, Switzerland) 20201027 11

In this work, the QuEChERS method coupled to liquid chromatography-time-of flight-mass spectrometry and gas chromatography-triple quadrupole-mass spectrometry were applied for the evaluation of pesticide residues and risk assessment in red wines. The methodologies were successfully validated for 173 pesticides. Recovery values were in the range 75-100% for almost all pesticides and limits of quantification were between 2.60 and 21.39 µg/kg, which are in good agreement with the maximum residue li ...[more]

PMID: 33121047

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Project description:BackgroundWolbachia pipientis is an endosymbiont bacterium that induces cytoplasmic incompatibility and inhibits arboviral replication in mosquitoes. This study aimed to assess Wolbachia prevalence and genetic diversity in different mosquito species from Cape Verde.MethodsMosquitoes were collected on six islands of Cape Verde and identified to species using morphological keys and PCR-based assays. Wolbachia was detected by amplifying a fragment of the surface protein gene (wsp). Multilocus sequence typing (MLST) was performed with five housekeeping genes (coxA, gatB, ftsZ, hcpA, and fbpA) and the wsp hypervariable region (HVR) for strain identification. Identification of wPip groups (wPip-I to wPip-V) was performed using PCR-restriction fragment length polymorphism (RFLP) assay on the ankyrin domain gene pk1.ResultsNine mosquito species were collected, including the major vectors Aedes aegypti, Anopheles arabiensis, Culex pipiens sensu stricto, and Culex quinquefasciatus. Wolbachia was only detected in Cx. pipiens s.s. (100% prevalence), Cx. quinquefasciatus (98.3%), Cx. pipiens/quinquefasciatus hybrids (100%), and Culex tigripes (100%). Based on the results of MLST and wsp hypervariable region typing, Wolbachia from the Cx. pipiens complex was assigned to sequence type 9, wPip clade, and supergroup B. PCR/RFLP analysis revealed three wPip groups in Cape Verde, namely wPip-II, wPip-III, and wPip-IV. wPip-IV was the most prevalent, while wPip-II and wPip-III were found only on Maio and Fogo islands. Wolbachia detected in Cx. tigripes belongs to supergroup B, with no attributed MLST profile, indicating a new strain of Wolbachia in this mosquito species.ConclusionsA high prevalence and diversity of Wolbachia was found in species from the Cx. pipiens complex. This diversity may be related to the mosquito's colonization history on the Cape Verde islands. To the best of our knowledge, this is the first study to detect Wolbachia in Cx. tigripes, which may provide an additional opportunity for biocontrol initiatives.

Dataset Information

Comparison of Pesticide Residue Levels in Red Wines from Canary Islands, Iberian Peninsula, and Cape Verde.

Publications

Comparison of Pesticide Residue Levels in Red Wines from Canary Islands, Iberian Peninsula, and Cape Verde.

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