Dataset Information

Visualization of antennal lobe glomeruli activated by nonappetitive D-limonene and appetitive 1-octen-3-ol odors via two types of olfactory organs in the blowfly Phormia regina.

ABSTRACT: Appetite or feeding motivation relies significantly on food odors. In the blowfly Phormia regina, feeding motivation for sucrose is decreased by the odor of D-limonene but increased by the odor of 1-octen-3-ol odor. These flies have antennal lobes (ALs) consisting of several tens of glomerular pairs as a primary olfactory center in the brain. Odor information from different olfactory organs-specifically, the antennae and maxillary palps-goes to the corresponding glomeruli. To investigate how odors differently affect feeding motivation, we identified the olfactory organs and glomeruli that are activated by nonappetitive and appetitive odors. We first constructed a glomerular map of the antennal lobe in P. regina. Anterograde fluorescence labeling of antennal and maxillary afferent nerves, both of which project into the contralateral and ipsilateral ALs, revealed differential staining in glomerular regions. Some of the axonal fiber bundles from the antennae and maxillary palps projected to the subesophageal ganglion (SOG). We visualized the activation of the glomeruli in response to odor stimuli by immunostaining phosphorylated extracellular signal-regulated kinase (pERK). We observed different glomerulus activation under different odor stimulations. Referring to our glomerular map, we determined that antennal exposure to D-limonene odor activated the DA13 glomeruli, while exposure of the maxillary palps to 1-octen-3-ol activated the MxB1 glomeruli. Our results indicated that a nonappetitive odor input from the antennae and an appetitive odor input from the maxillary palps activate different glomeruli in the different regions of ALs in the blowfly P. regina. Collectively, our findings suggest that compartmentalization of glomeruli in AL is essential for proper transmission of odor information.

SUBMITTER: Maeda T

PROVIDER: S-EPMC7694429 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Publications

Visualization of antennal lobe glomeruli activated by nonappetitive D-limonene and appetitive 1-octen-3-ol odors via two types of olfactory organs in the blowfly Phormia regina.

Maeda Toru T Nisimura Tomoyosi T Habe Shunnya S Uebi Tatsuya T Ozaki Mamiko M

Zoological letters 20201127 1

Appetite or feeding motivation relies significantly on food odors. In the blowfly Phormia regina, feeding motivation for sucrose is decreased by the odor of D-limonene but increased by the odor of 1-octen-3-ol odor. These flies have antennal lobes (ALs) consisting of several tens of glomerular pairs as a primary olfactory center in the brain. Odor information from different olfactory organs-specifically, the antennae and maxillary palps-goes to the corresponding glomeruli. To investigate how odo ...[more]

PMID: 33292700

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Project description:The flowers of different plant species have diverse scents with varied chemical compositions. Hence, every floral scent does not uniformly affect insect feeding preferences. The blowfly, Phormia regina, is a nectar feeder, and when a fly feeds on flower nectar, its olfactory organs, antennae, and maxillary palps are exposed to the scent. Generally, feeding preference is influenced by food flavor, which relies on both taste and odor. Therefore, the flies perceive the sweet taste of nectar and the particular scent of the flower simultaneously, and this olfactory information affects their feeding preference. Here, we show that the floral scents of 50 plant species have various effects on their sucrose feeding motivation, which was evaluated using the proboscis extension reflex (PER). Those floral scents were first categorized into three groups, based on their effects on the PER threshold sucrose concentration, which indicates whether a fly innately dislikes, ignores, or likes the target scent. Moreover, memory of olfactory experience with those floral scents during sugar feeding influenced the PER threshold. After feeding on sucrose solutions flavored with floral scents for 5 days, the scents did not consistently show the previously observed effects. Considering such empirical effects of scents on the PER threshold, we categorized the effects of the 50 tested floral scents on feeding preference into 16 of all possible 27 theoretical types. We then conducted the same experiments with flies whose antennae or maxillary palps were ablated prior to PER test in a fly group naïve to floral scents and prior to the olfactory experience during sugar feeding in the other fly group in order to test how these organs were involved in the effect of the floral scent. The results suggested that olfactory inputs through these organs play different roles in forming or modifying feeding preferences. Thus, our study contributes to an understanding of underlying mechanisms associated with the convergent processing of olfactory inputs with taste information, which affects feeding preference or appetite.

Dataset Information

Visualization of antennal lobe glomeruli activated by nonappetitive D-limonene and appetitive 1-octen-3-ol odors via two types of olfactory organs in the blowfly Phormia regina.

Publications

Visualization of antennal lobe glomeruli activated by nonappetitive D-limonene and appetitive 1-octen-3-ol odors via two types of olfactory organs in the blowfly Phormia regina.

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