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Impacts of different operational temperatures and organic loads in anaerobic co-digestion of food waste and sewage sludge on the fate of SARS-CoV-2.

ABSTRACT: The impacts of different operational temperatures, and organic load (OL) on the fate of SARS-CoV-2 during the anaerobic co-digestion of food waste (FW) and sewage sludge (SS) was evaluated. The lab-scaled batch reactors (i.e. R1-R7) were performed under psychrophilic, mesophilic, and thermophilic conditions and the OL of systems was 1.5, 3.5, 6 gVS/L. The performance parameters showed that at higher OL the stability of systems failed and low biogas was produced. In contrast, increasing of operational temperature of systems induced more biogas generation due to the increment of metabolic activity of bacteria. Therefore, R1-R7 achieved biogas yield of 202.5, 249, 187, 260, 246, 163, and 300 mL/gVS respectively. Both SARS-CoV-2 genes i.e. ORF1ab, and N genes were detected in the effluent of psychrophilic reactors i.e. R1, and R2, with a total concentration of 46 × 10³, and 11 × 10³ copies/L respectively. In R3, no viral genes were observed, when the VFAs was accumulated up to 2000 mg/L and caused a pH drop to 5.6. At the mesophilic condition, the viral concentration was significantly declined, and no viral genes were observed at an OL of 3.5 gVS/L. Furthermore, the synergistic effect of temperature and accumulation of intermediate metabolites provided a sever condition for SARS-CoV-2 survival at an operational temperature and OL of 50 °C, and 1.5 gVS/L respectively.

SUBMITTER: Bardi MJ

PROVIDER: S-EPMC7694560 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Impacts of different operational temperatures and organic loads in anaerobic co-digestion of food waste and sewage sludge on the fate of SARS-CoV-2.

Bardi Mohammad Javad MJ Oliaee Mohammad Amin MA

Process safety and environmental protection : transactions of the Institution of Chemical Engineers, Part B 20201127

The impacts of different operational temperatures, and organic load (OL) on the fate of SARS-CoV-2 during the anaerobic co-digestion of food waste (FW) and sewage sludge (SS) was evaluated. The lab-scaled batch reactors (i.e. R1-R7) were performed under psychrophilic, mesophilic, and thermophilic conditions and the OL of systems was 1.5, 3.5, 6 gVS/L. The performance parameters showed that at higher OL the stability of systems failed and low biogas was produced. In contrast, increasing of operat ...[more]

PMID: 33262558

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Project description:Anaerobic co-digestion (Co-AD) is used to increase the effectiveness of anaerobic digestion (AD) using local "wastes", adding economic and environmental benefits. Since system stability is of existential importance for the operation of wastewater treatment plants, thorough testing of potential co-substrates and their effects on the respective community and system performance is crucial for understanding and utilizing Co-AD to its best capacity. Food waste (FW) and canola lecithin (CL) were tested in mesophilic, lab-scale, semi-continuous reactors over a duration of 120 days with stepwise increased substrate addition. Key performance indicators (biogas, total/volatile solids, fatty acids) were monitored and combined with 16S-rRNA amplicon sequencing to assess the impact of co-substrate addition on reactor performance and microbial community composition (MCC). Additionally, the latter was then compared with natural shifts occurring in the wastewater treatment plant (WWTP, source) at the same time. An almost linear increase in biogas production with both co-substrates at an approximate 1:1 ratio with the organic loading rate (OLR) was observed. The MCCs in both experiments were mostly stable, but also prone to drift over time. The FW experiment MCC more closely resembled the original WWTP community and the observed shifts indicated high levels of functional redundancy. Exclusive to the CL co-substrate, a clear selection for a few operational taxonomic units (OTUs) was observed. There was little evidence for a persistent invasion and establishment of microorganisms from typical primary substrates into the stable resident community of the reactors, which is in line with earlier findings that suggested that the inoculum and history mostly define the MCC. However, external factors may still tip the scales in favor of a few r-strategists (e.g., Prolixibacter) in an environment that otherwise favors K-strategists, which may in fact also be recruited from the primary substrate (Trichococcus). In our study, specialization and diversity loss were also observed in response to the addition of the highly specialized CL, which in turn, may have adverse effects on the system's stability and reduced resilience and recovery.

Project description:BackgroundMost of the organic content of waste activated sludge (WAS) comprises microbial cells hard to degrade, which must be pre-treated for energy recovery by anaerobic digestion (AD). Electrooxidation pre-treatment (EOP) with boron-doped diamond (BDD) electrode have been considered a promising novel technology that increase hydrolysis rate, by the disintegrating cell walls from WAS. Although electrochemical oxidation could efficiently solubilize organic substances of macromolecules, limited reports are available on EOP of WAS for improving AD. In this endeavour, the mathematical optimization study and the energy analysis of the effects of initial total solids concentrations [TS] of WAS and current density (CD) during EOP on the methane production and removal of chemical oxygen demand (COD) and volatile solids (VS) were investigated. Because limited reports are available on EOP of WAS for improving biogas production, it is not well understood; however, it has started to attract interest of scientists and engineers.ResultsIn the present work, the energy recovery as biogas and WAS conversion were comprehensively affected by CD and [TS], in an integrated EOP and AD system. When working with WAS at 3% of [TS] pre-treated at current density of 24.1 mA/cm2, the highest COD and VS removal were achieved, making it possible to obtain the maximum methane (CH4) production of 305 N-L/kg VS and a positive energy balance of 1.67 kWh/kg VS. Therefore, the current densities used in BDD electrode are adequate to produce the strong oxidant (hydroxyl radical, ·OH) on the electrode surface, allow the oxidation of organic compounds that favours the solubilization of particulate matter and VS from WAS.ConclusionsThe improvement of VS removal and COD solubilization were due to the effects of pre-treatments, which help to break down the microbial cells for faster subsequent degradation; this allows a decomposition reaction that leads to biodegrade more compounds during AD. The balance was positive, suggesting that even without any optimization the energy used as electricity could be recovered from the increased methane production. It is worth noting that this kind of analysis have not been sufficiently studied so far. It is therefore important to understand how operational parameters can influence the pre-treatment and AD performances. The current study highlights that the mathematical optimization and energy analysis can make the whole process more convenient and feasible.

Dataset Information

Impacts of different operational temperatures and organic loads in anaerobic co-digestion of food waste and sewage sludge on the fate of SARS-CoV-2.

Publications

Impacts of different operational temperatures and organic loads in anaerobic co-digestion of food waste and sewage sludge on the fate of SARS-CoV-2.

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