Dataset Information

Effect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses.

ABSTRACT: Background: Omeprazole administration is associated with changes in gastric and fecal microbiota and increased incidence of Clostridioides difficile enterocolitis in humans and dogs.

Hypothesis/objectives: Study purpose was to assess the effect of omeprazole on gastric glandular and fecal microbiota in healthy adult horses.

Animals: Eight healthy horses stabled on straw and fed 100% haylage.

Methods: Prospective controlled study. Transendoscopic gastric glandular biopsies, gastric fluid, and fecal samples were obtained from each horse twice at a 7-day interval before the administration of omeprazole. Samples were taken on the same horses before and after a 7-day administration of omeprazole (4 mg/kg PO q24h). pH was assessed on fresh gastric fluid and other samples were kept at -20°C until analysis. Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing from feces and gastric glandular biopsies. Analysis of alpha, beta diversity, and comparison between time points were performed with MOTHUR and results were considered significant when P?
Results: Gastric pH increased significantly after 7?days of omeprazole administration (P = .006). Omeprazole did not induce significant major changes in composition of fecal or gastric glandular microbiota, however, after administration, certain microbial genera became more predominant in the gastric glandular mucosa (lower Simpson's evenness, P = .05). Only the genus Clostridium sensu strictu_1 had a significant shift in the glandular gastric mucosa after omeprazole administration (P = .002). No population shifts were observed in feces.

Conclusions and clinical importance: Oral administration of omeprazole could have fewer effects in gastrointestinal microbiota in the horse compared to other species.

SUBMITTER: Cerri S

PROVIDER: S-EPMC7694827 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Effect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses.

Cerri Simona S Taminiau Bernard B de Lusancay Alexandra Hache-Carré AH Lecoq Laureline L Amory Hélène H Daube Georges G Cesarini Carla C

Journal of veterinary internal medicine 20201016 6

<h4>Background</h4>Omeprazole administration is associated with changes in gastric and fecal microbiota and increased incidence of Clostridioides difficile enterocolitis in humans and dogs.<h4>Hypothesis/objectives</h4>Study purpose was to assess the effect of omeprazole on gastric glandular and fecal microbiota in healthy adult horses.<h4>Animals</h4>Eight healthy horses stabled on straw and fed 100% haylage.<h4>Methods</h4>Prospective controlled study. Transendoscopic gastric glandular biopsie ...[more]

PMID: 33063923

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Project description:The administration of an oral probiotic has been demonstrated to impact oral microbial diversity in humans but has not been examined in canines. The objective of this study was to test the hypothesis that oral probiotic administration would impact the oral microbiota of canines compared to control. Working canines in training (n = 13) were assigned to Test or Control groups and acclimated to one of three commercially available study diets utilizing common protein sources (Purina Pro Plan Savor lamb, Purina Pro Plan Sport chicken, Purina Pro Plan Focus salmon) for a minimum of 30 days prior to initiation of the study. Following acclimation, dogs in the Test group began a daily regimen of oral probiotic (Fortiflora® Purina, St. Louis, MO) top-dressed on their midday feeding. Control dogs received their midday feeding with no probiotic. All dogs were sampled once weekly via oral pediatric swabs across the 7-week study. Next generation sequencing (Illumina, MiSeq) was utilized to develop microbial profiles specific to treatment, diet, and time. Bacterial composition was dominated by eight phyla (Proteobacteria 43.8%, Bacteroidetes 22.5%, Firmicutes 18.9%, Actinobacteria 6.1%, Fusobacteria 3.6%, Gracilibacteria 2.1%, SR1 Absconditabacteria 1.5%, and Saccharibacteria 1.3%) representing more than 99% of the relative abundance of the microbial composition. Probiotic administration failed to impact relative abundance at any taxonomic level (P > 0.05). Similarly, no effect on the oral microbiota was measured for diet (P > 0.05). Comparison using a Jaccard Index demonstrate a consistent microbial profile over the 7-week study with no impact evidenced by study week (P = 0.19). The data also revealed a profile of ubiquitous taxa that were present across all dogs and all samples regardless of breed, sex, diet, treatment or other factors. These genera include Actinomyces, Corynebacterium, Capnocytophaga, Flavobacterium, Gemella, Abiotrophia, Streptococcus, and Frederiksenia. These data demonstrate the stability of canine oral microbiota over time.

Dataset Information

Effect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses.

Publications

Effect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses.

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