Dataset Information

Artificial Light at Night Increases Aedes aegypti Mosquito Biting Behavior with Implications for Arboviral Disease Transmission.

ABSTRACT: Aedes aegypti mosquito is a major vector of arboviral disease. Here, we report that the biting behavior of normally daytime active anthropophilic Ae. aegypti mosquitoes on human hosts is abnormally increased at night following exposure to artificial light at night (ALAN). Biting was examined using a human host assay where caged mosquitoes were exposed to a human arm and blood-feeding measured. Mosquitoes were tested during the daytime, nighttime, or challenged with ALAN. As predicted from the Ae. aegypti diel/circadian biting cycle, maximal biting occurred during daytime and lowest level occurred at night. Biting in the ALAN group was increased compared with time-matched nighttime controls. These data reveal that exposure to ALAN increases nocturnal blood-feeding behavior. This finding highlights the concern that globally increasing levels of light pollution could be impacting arboviral disease transmission, such as dengue fever and Zika, and has implications for application of countermeasures for mosquito vector control.

SUBMITTER: Rund SSC

PROVIDER: S-EPMC7695055 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Artificial Light at Night Increases Aedes aegypti Mosquito Biting Behavior with Implications for Arboviral Disease Transmission.

Rund Samuel S C SSC Labb Laura F LF Benefiel Owen M OM Duffield Giles E GE

The American journal of tropical medicine and hygiene 20201013 6

Aedes aegypti mosquito is a major vector of arboviral disease. Here, we report that the biting behavior of normally daytime active anthropophilic Ae. aegypti mosquitoes on human hosts is abnormally increased at night following exposure to artificial light at night (ALAN). Biting was examined using a human host assay where caged mosquitoes were exposed to a human arm and blood-feeding measured. Mosquitoes were tested during the daytime, nighttime, or challenged with ALAN. As ...[more]

PMID: 33069264

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Project description:Biomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898 proteins from the Yellow Fever and dengue virus vector, Aedes aegypti, during ageing. By applying a mixed-effects model of protein expression, we identified five common patterns of abundance change during ageing and demonstrated an age-related decrease in variance for four of these. This supported a search for specific proteins with abundance changes that remain tightly associated with ageing for use as ageing biomarkers. Using MALDI-TOF/TOF mass spectrometry we identified ten candidate proteins that satisfied strict biomarker discovery criteria (identified in two out of three multivariate analysis procedures and in two cohorts of mosquitoes). We validated the abundances of the four most suitable candidates (Actin depolymerising factor; ADF, Eukaryotic initiation factor 5A; eIF5A, insect cuticle protein Q17LN8, and Anterior fat body protein; AFP) using semi-quantitative Western analysis of individual mosquitoes of six ages. The redox-response protein Manganese superoxide dismutase (SOD2) and electron shuttling protein Electron transfer oxidoreductase (ETO) were subject to post-translational modifications affecting their charge states with potential effects on function. For the four candidates we show remarkably consistent decreases in abundance during ageing, validating initial selections. In particular, the abundance of AFP is an ideal biomarker candidate for whether a female mosquito has lived long enough to be capable of dengue virus transmission. We have demonstrated proteins to be a suitable class of ageing biomarkers in mosquitoes and have identified candidates for epidemiological studies of dengue and the evaluation of new disease reduction projects targeting mosquito longevity.

Dataset Information

Artificial Light at Night Increases Aedes aegypti Mosquito Biting Behavior with Implications for Arboviral Disease Transmission.

Publications

Artificial Light at Night Increases <i>Aedes aegypti</i> Mosquito Biting Behavior with Implications for Arboviral Disease Transmission.

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