Dataset Information

Mediterranean Fruit Fly Ceratitis capitata (Diptera: Tephritidae) Eggs and Larvae Responses to a Low-Oxygen/High-Nitrogen Atmosphere.

ABSTRACT: The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the most damaging horticultural insect pests. This study used a low-oxygen/high-nitrogen bioassay to control C. capitata. Two low-oxygen treatments were applied (0.5% O₂ + 99.5 N₂ and 5% O₂ + 95% N₂) to C. capitata eggs and 1st, 2nd and 3rd instar larvae from 0 to nine days on a carrot diet at 25 °C; 70-75% RH. The pupariation, adult emergence, and sex ratios of survived flies were examined. The results demonstrate that increased mortality of all tested life stages correlated with increased exposure times at both levels of low-oxygen treatments. Complete control of eggs was achieved after eight days and nine days for larvae using 0.5% O₂ at 25 °C; 70-75% RH. The 3rd instar was the most tolerant stage, while the egg was the most susceptible stage to the low-oxygen environment. There were no significant differences in sex ratios between emerged adults after low-oxygen and control treatments. The present work demonstrates and confirms the mortalities of C. capitata caused by low-oxygen treatment, which may help develop new postharvest strategies to control this destructive fruit fly pest.

SUBMITTER: Al-Behadili FJM

PROVIDER: S-EPMC7696186 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Mediterranean Fruit Fly Ceratitis capitata (Diptera: Tephritidae) Eggs and Larvae Responses to a Low-Oxygen/High-Nitrogen Atmosphere.

Al-Behadili Farhan J M FJM Agarwal Manjree M Xu Wei W Ren Yonglin Y

Insects 20201113 11

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the most damaging horticultural insect pests. This study used a low-oxygen/high-nitrogen bioassay to control C. capitata. Two low-oxygen treatments were applied (0.5% O2 + 99.5 N2 and 5% O2 + 95% N2) to C. capitata eggs and 1st, 2nd and 3rd instar larvae from 0 to nine days on a carrot diet at 25 °C; 70-75% RH. The pupariation, adult emerg ...[more]

PMID: 33203006

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Project description:The Mediterranean fruit fly, Ceratitis capitata (medfly), is an extremely invasive agricultural pest due to its extremely wide host range and its ability to adapt to a broad range of climatic conditions and habitats. Chemosensory behaviour plays an important role in many crucial stages in the life of this insect, such as the detection of pheromone cues during mate pursuit and odorants during host plant localisation. Thus, the analysis of the chemosensory gene repertoire is an important step for the interpretation of the biology of this species and consequently its invasive potential. Moreover, these genes may represent ideal targets for the development of novel, effective control methods and pest population monitoring systems. Expressed sequence tag libraries from C. capitata adult heads, embryos, male accessory glands and testes were screened for sequences encoding putative odorant binding proteins (OBPs). A total of seventeen putative OBP transcripts were identified, corresponding to 13 Classic, three Minus-C and one Plus-C subfamily OBPs. The tissue distributions of the OBP transcripts were assessed by RT-PCR and a subset of five genes with predicted proteins sharing high sequence similarities and close phylogenetic affinities to Drosophila melanogaster pheromone binding protein related proteins (PBPRPs) were characterised in greater detail. Real Time quantitative PCR was used to assess the effects of maturation, mating and time of day on the transcript abundances of the putative PBPRP genes in the principal olfactory organs, the antennae, in males and females. The results of the present study have facilitated the annotation of OBP genes in the recently released medfly genome sequence and represent a significant contribution to the characterisation of the medfly chemosensory repertoire. The identification of these medfly OBPs/PBPRPs permitted evolutionary and functional comparisons with homologous sequences from other tephritids of the genera Bactrocera and Rhagoletis.

Dataset Information

Mediterranean Fruit Fly Ceratitis capitata (Diptera: Tephritidae) Eggs and Larvae Responses to a Low-Oxygen/High-Nitrogen Atmosphere.

Publications

Mediterranean Fruit Fly <i>Ceratitis capitata</i> (Diptera: Tephritidae) Eggs and Larvae Responses to a Low-Oxygen/High-Nitrogen Atmosphere.

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