Cannabinoid Type 1 Receptor is Undetectable in Rodent and Primate Cerebral Neural Stem Cells but Participates in Radial Neuronal Migration.

ABSTRACT: Cannabinoid type 1 receptor (CB₁R) is expressed and participates in several aspects of cerebral cortex embryonic development as demonstrated with whole-transcriptome mRNA sequencing and other contemporary methods. However, the cellular location of CB₁R, which helps to specify molecular mechanisms, remains to be documented. Using three-dimensional (3D) electron microscopic reconstruction, we examined CB₁R immunolabeling in proliferating neural stem cells (NSCs) and migrating neurons in the embryonic mouse (Mus musculus) and rhesus macaque (Macaca mulatta) cerebral cortex. We found that the mitotic and postmitotic ventricular and subventricular zone (VZ and SVZ) cells are immunonegative in both species while radially migrating neurons in the intermediate zone (IZ) and cortical plate (CP) contain CB₁R-positive intracellular vesicles. CB₁R immunolabeling was more numerous and more extensive in monkeys compared to mice. In CB₁R-knock out mice, projection neurons in the IZ show migration abnormalities such as an increased number of lateral processes. Thus, in radially migrating neurons CB₁R provides a molecular substrate for the regulation of cell movement. Undetectable level of CB₁R in VZ/SVZ cells indicates that previously suggested direct CB₁R-transmitted regulation of cellular proliferation and fate determination demands rigorous re-examination. More abundant CB₁R expression in monkey compared to mouse suggests that therapeutic or recreational cannabis use may be more distressing for immature primate neurons than inferred from experiments with rodents.

SUBMITTER: Morozov YM 

PROVIDER: S-EPMC7696736 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature