Unknown

Dataset Information

0

Mitochondrial Ca2+ Dynamics in MCU Knockout C. elegans Worms.


ABSTRACT: Mitochondrial [Ca2+] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca2+ overload. This regulation depends on Ca2+ entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca2+ uniporter (MCU). Here, we have studied the mitochondrial Ca2+ dynamics in control and MCU-defective C. elegans worms in vivo, by using worms expressing mitochondrially-targeted YC3.60 yellow cameleon in pharynx muscle. Our data show that the small mitochondrial Ca2+ oscillations that occur during normal physiological activity of the pharynx were very similar in both control and MCU-defective worms, except for some kinetic differences that could mostly be explained by changes in neuronal stimulation of the pharynx. However, direct pharynx muscle stimulation with carbachol triggered a large and prolonged increase in mitochondrial [Ca2+] that was much larger in control worms than in MCU-defective worms. This suggests that MCU is necessary for the fast mitochondrial Ca2+ uptake induced by large cell stimulations. However, low-amplitude mitochondrial Ca2+ oscillations occurring under more physiological conditions are independent of the MCU and use a different Ca2+ pathway.

SUBMITTER: Alvarez-Illera P 

PROVIDER: S-EPMC7696937 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mitochondrial Ca<sup>2+</sup> Dynamics in MCU Knockout <i>C. elegans</i> Worms.

Álvarez-Illera Pilar P   García-Casas Paloma P   Fonteriz Rosalba I RI   Montero Mayte M   Alvarez Javier J  

International journal of molecular sciences 20201116 22


Mitochondrial [Ca<sup>2+</sup>] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca<sup>2+</sup> overload. This regulation depends on Ca<sup>2+</sup> entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca<sup>2+</sup> uniporter (MCU). Here, we have studied the mitochondrial Ca<sup>2+</sup> dynamics in control and MCU-defective <i>C. elegans</i> wor  ...[more]

Similar Datasets

| S-EPMC4722812 | biostudies-literature
| S-EPMC9614509 | biostudies-literature
| S-EPMC3464549 | biostudies-literature
| S-EPMC7525533 | biostudies-literature
| S-EPMC3400633 | biostudies-literature
| S-EPMC1305047 | biostudies-literature
| S-EPMC3988891 | biostudies-literature
| S-EPMC6378946 | biostudies-literature
| S-EPMC5760244 | biostudies-literature
| S-EPMC4734683 | biostudies-literature