Dataset Information

Antioxidant and Cytoprotective Effect of Quinoa (Chenopodium quinoa Willd.) with Pressurized Hot Water Extraction (PHWE).

ABSTRACT: Quinoa is widely noted for its nutritional value. The seed is the main edible part of the plant and exists in at least three different colors: white, red and black. This study utilized a pressurized hot water extraction (PHWE) for the extraction of phytochemicals from quinoa. Chemical fingerprints with LC/UV and LC/MS using a targeted approach and pattern recognition tools were used to evaluate the quinoa extracts. The antioxidant properties for various types of quinoa were evaluated using DPPH assay, ABTS assay and the cytoprotective effect of quinoa extracts were investigated in HMEC-1 cell line. Distinctive chemical profiles obtained from black and red quinoa were well correlated with the antioxidant activities and cytoprotective effects. The combination of PHWE, chemical standardization with LC/UV and LC/MS, pattern recognition tools and biological assay provided an approach for the evaluation and eventual production of quinoa extracts for functional food.

SUBMITTER: Ong ES

PROVIDER: S-EPMC7697190 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Antioxidant and Cytoprotective Effect of Quinoa (<i>Chenopodium quinoa Willd</i>.) with Pressurized Hot Water Extraction (PHWE).

Ong Eng Shi ES Pek Charlene Jia Ning CJN Tan Joseph Choon Wee JCW Leo Chen Huei CH

Antioxidants (Basel, Switzerland) 20201111 11

Quinoa is widely noted for its nutritional value. The seed is the main edible part of the plant and exists in at least three different colors: white, red and black. This study utilized a pressurized hot water extraction (PHWE) for the extraction of phytochemicals from quinoa. Chemical fingerprints with LC/UV and LC/MS using a targeted approach and pattern recognition tools were used to evaluate the quinoa extracts. The antioxidant properties for various types of quinoa were evaluated using DPPH ...[more]

PMID: 33187302

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Project description:The total area under quinoa (Chenopodium quinoa Willd.) cultivation and the consumption of its grain have increased in recent years because of its nutritional properties and ability to grow under adverse conditions, such as drought. Climate change scenarios predict extended periods of drought and this has emphasized the need for new crops that are tolerant to these conditions. The main goal of this work was to evaluate crop yield and quality parameters and to characterize the physiology of two varieties of quinoa grown under water deficit in greenhouse conditions. Two varieties of quinoa from the Chilean coast (Rainbow) and altiplano (Illpa) were used, grown under full irrigation or two different levels of water deficit applied during the grain filling period. There were no marked differences in yield and quality parameters between treatments, but the root biomass was higher in plants grown under severe water deficit conditions compared to control. Photosynthesis, transpiration and stomatal conductance decreased with increased water stress in both cultivars, but the coastal variety showed higher water use efficiency and less discrimination of 13C under water deficit. This response was associated with greater root development and a better stomatal opening adjustment, especially in the case of Rainbow. The capacity of Rainbow to increase its osmoregulant content (compounds such as proline, glutamine, glutamate, K and Na) could enable a potential osmotic adjustment in this variety. Moreover, the lower stomatal opening and transpiration rates were also associated with higher leaf ABA concentration values detected in Rainbow. We found negative logarithmic relationships between stomatal conductance and leaf ABA concentration in both varieties, with significant R2 values of 0.50 and 0.22 in Rainbow and Illpa, respectively. These moderate-to-medium values suggest that, in addition to ABA signaling, other causes for stomatal closure in quinoa under drought such as hydraulic regulation may play a role. In conclusion, this work showed that two quinoa cultivars use different strategies in the face of water deficit stress, and these prevent decreases in grain yield and quality under drought conditions.

Dataset Information

Antioxidant and Cytoprotective Effect of Quinoa (Chenopodium quinoa Willd.) with Pressurized Hot Water Extraction (PHWE).

Publications

Antioxidant and Cytoprotective Effect of Quinoa (<i>Chenopodium quinoa Willd</i>.) with Pressurized Hot Water Extraction (PHWE).

Similar Datasets

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