Dataset Information

Identification of Novel Astroviruses in the Gastrointestinal Tract of Domestic Cats.

ABSTRACT: Astroviruses, isolated from numerous avian and mammalian species including humans, are commonly associated with enteritis and encephalitis. Two astroviruses have previously been identified in cats, and while definitive evidence is lacking, an association with enteritis is suggested. Using metagenomic next-generation sequencing of viral nucleic acids from faecal samples, we identified two novel feline astroviruses termed Feline astrovirus 3 and 4. These viruses were isolated from healthy shelter-housed kittens (Feline astrovirus 3; 6448 bp) and from a kitten with diarrhoea that was co-infected with Feline parvovirus (Feline astrovirus 4, 6549 bp). Both novel astroviruses shared a genome arrangement of three open reading frames (ORFs) comparable to that of other astroviruses. Phylogenetic analysis of the concatenated ORFs, ORF1a, ORF1b and capsid protein revealed that both viruses were phylogenetically distinct from other feline astroviruses, although their precise evolutionary history could not be accurately determined due to a lack of resolution at key nodes. Large-scale molecular surveillance studies of healthy and diseased cats are needed to determine the pathogenicity of feline astroviruses as single virus infections or in co-infections with other enteric viruses.

SUBMITTER: Brussel KV

PROVIDER: S-EPMC7697530 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Identification of Novel Astroviruses in the Gastrointestinal Tract of Domestic Cats.

Brussel Kate Van KV Wang Xiuwan X Shi Mang M Carrai Maura M Li Jun J Martella Vito V Beatty Julia A JA Holmes Edward C EC Barrs Vanessa R VR

Viruses 20201112 11

Astroviruses, isolated from numerous avian and mammalian species including humans, are commonly associated with enteritis and encephalitis. Two astroviruses have previously been identified in cats, and while definitive evidence is lacking, an association with enteritis is suggested. Using metagenomic next-generation sequencing of viral nucleic acids from faecal samples, we identified two novel feline astroviruses termed Feline astrovirus 3 and 4. These viruses were isolated from healthy shelter- ...[more]

PMID: 33198334

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Dataset Information

Identification of Novel Astroviruses in the Gastrointestinal Tract of Domestic Cats.

Publications

Identification of Novel Astroviruses in the Gastrointestinal Tract of Domestic Cats.

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