Identification and Characterization of the Intra-Articular Microbiome in the Osteoarthritic Knee.

ABSTRACT: Osteoarthritis (OA) is the most common joint disorder in the United States, and the gut microbiome has recently emerged as a potential etiologic factor in OA development. Recent studies have shown that a microbiome is present at joint synovia. Therefore, we aimed to characterize the intra-articular microbiome within osteoarthritic synovia and to illustrate its role in OA disease progression. RNA-sequencing data from OA patient synovial tissue was aligned to a library of microbial reference genomes to identify microbial reads indicative of microbial abundance. Microbial abundance data of OA and normal samples was compared to identify differentially abundant microbes. We computationally explored the correlation of differentially abundant microbes to immunological gene signatures, immune sign

SUBMITTER: Tsai JC 

PROVIDER: S-EPMC7697780 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature