Self-Assembling ?-Glucan Nanomedicine for the Delivery of siRNA.

ABSTRACT: We aimed to design and manufacture a transporter capable of delivering small interfering RNAs (siRNAs) into the skin without causing any damage. ?-glucans are unique chiral polysaccharides with well-defined immunological properties and supramolecular wrapping ability. However, the chiral properties of these polymers have hardly been applied in drug delivery systems. In this study, ?-glucan nanoparticles were designed and manufactured to deliver genetic material to the target cells. The ?-glucan molecules were self-assembled with an siRNA into nanoparticles of 300-400 nm in diameter via a conformational transition process, in order to construct a gene delivery system. The assembled gene nanocarriers were associated with high gene-loading ability. The expression and efficiency of siRNA were verified after its delivery via ?-glucan. Our results provide evidence that ?-glucan nanoparticles can be effectively used to deliver siRNA into the cells.

SUBMITTER: Lee K 

PROVIDER: S-EPMC7698166 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature