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Post-Anthesis Water-stressed Barley Maintains Grain Specific Weight Through Altered Grain Composition and Plant Architecture.

ABSTRACT: Specific weight (SW) is a long-established measure used as a malting quality specification in barley, with an increased SW thought to result in a higher malt output. Specific weight is a product of individual grain density as determined by grain composition and structure, and grain packing efficiency in a container as determined by grain dimensions. We investigated the effect of moderate but prolonged post-anthesis water stress on barley plant and grain development using pots of cultivars with a known range of SWs to explore how altering plant growth influence SW. Water stress was expected to influence these grain characteristics through decreased photosynthetic capacity. We demonstrated that SW was maintained under water stress conditions through compensatory mechanisms such as increased tiller mortality which preserved grain physical parameters on the main shoots. However, water stress significantly affected plant development by reducing not only ear number and yield, but also grain filling duration, plant biomass and ear length. Grain composition was also altered, with water-stressed plants having reduced carbon:nitrogen. Therefore, although SW can be conserved under water-stressed conditions, grain composition and plant development are altered, producing smaller harvests with higher grain nitrogen content. This would result in bulks of malting barley having different malt outputs despite having the same SW.

SUBMITTER: Hoyle A

PROVIDER: S-EPMC7698198 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Post-Anthesis Water-stressed Barley Maintains Grain Specific Weight Through Altered Grain Composition and Plant Architecture.

Hoyle Aaron A Brennan Maree M Rees Logan L Jackson Gail E GE Hoad Stephen P SP

Plants (Basel, Switzerland) 20201113 11

Specific weight (SW) is a long-established measure used as a malting quality specification in barley, with an increased SW thought to result in a higher malt output. Specific weight is a product of individual grain density as determined by grain composition and structure, and grain packing efficiency in a container as determined by grain dimensions. We investigated the effect of moderate but prolonged post-anthesis water stress on barley plant and grain development using pots of cultivars with a ...[more]

PMID: 33202786

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Project description:BACKGROUND:High post-anthesis (p.a) temperatures reduce mature grain weights in wheat and other cereals. However, the causes of this reduction are not entirely known. Control of grain expansion by the maternally derived pericarp of the grain has previously been suggested, although this interaction has not been investigated under high p.a. temperatures. Down-regulation of pericarp localised genes that regulate cell wall expansion under high p.a. temperatures may limit expansion of the encapsulated endosperm due to a loss of plasticity in the pericarp, reducing mature grain weight. Here the effect of high p.a. temperatures on the transcriptome of the pericarp and endosperm of the wheat grain during early grain-filling was investigated via RNA-Seq and is discussed alongside grain moisture dynamics during early grain development and mature grain weight. RESULTS:High p.a. temperatures applied from 6-days after anthesis (daa) and until 18daa reduced the grain's ability to accumulate water, with total grain moisture and percentage grain moisture content being significantly reduced from 14daa onwards. Mature grain weight was also significantly reduced by the same high p.a. temperatures applied from 6daa for 4-days or more, in a separate experiment. Comparison of our RNA-Seq data from whole grains, with existing data sets from isolated pericarp and endosperm tissues enabled the identification of subsets of genes whose expression was significantly affected by high p.a. temperature and predominantly expressed in either tissue. Hierarchical clustering and gene ontology analysis resulted in the identification of a number of genes implicated in the regulation of cell wall expansion, predominantly expressed in the pericarp and significantly down-regulated under high p.a. temperatures, including endoglucanase, xyloglucan endotransglycosylases and a ?-expansin. An over-representation of genes involved in the 'cuticle development' functional pathway that were expressed in the pericarp and affected by high p.a. temperatures was also observed. CONCLUSIONS:High p.a. temperature induced down-regulation of genes involved in regulating pericarp cell wall expansion. This concomitant down-regulation with a reduction in total grain moisture content and grain weight following the same treatment period, adds support to the theory that high p.a. temperatures may cause a reduction in mature grain weight as result of decreased pericarp cell wall expansion.

Project description:Introduction Approximately 50% of irrigation water is saved during drip-irrigation of rice, which has tremendous potential for water-saving agriculture, particularly in areas where water resources are scarce. However, the grain yield and quality of drip-irrigated rice are adversely affected. Methods In this study, we investigated the effects of different irrigation strategies on the grain yield and quality of drip-irrigated rice using field experiments. Four irrigation treatments were studied: whole growing season flooding (FI), whole growing season normal drip irrigation (DI, soil relative moisture (RSM) was maintained in the range of 90-100%), pre-anthesis drip irrigation and post-anthesis water stress (SAF, the RSM was maintained in the range of 80-90% after anthesis), pre-anthesis drip irrigation, and post-anthesis flooding (FAF). Results The results showed that grain yield, harvest index, seed setting rate and 1000 grain weight in DI and SAF were significantly lower than in FI and FAF. These parameters were not significantly different between FI and FAF but were significantly greater in DI than in SAF. Compared with FI and FAF, the source capacity, source activity time, and sink activity of DI and SAF decreased, and the sink-source difference increased. The sink-source difference had a significant negative correlation with rice yield and 1000 grain weight. The activities of ADP-glucose pyrophosphorylase, starch branching enzyme, and amylopectin content in grains in the middle panicles of FAF were significantly higher than those of DI and SAF. SAF resulted in increased amylose/amylopectin ratio and total protein content in grains but decreased proportion of glutenin in total protein. Irrigation after anthesis of drip-irrigated rice narrowed the difference between sink sources in rice plants, increased the grain yield and harvest index by 29.2% and 11%, respectively, compared to DI, increased water productivity by 19% compared to FI, and improved the grain quality of drip-irrigated rice. Discussion This study highlights that post-anthesis sufficient irrigation of drip-irrigated rice plays a positive role in maintaining the source-sink balance. This study serves as a foundation for the development of more effective rice farming methods that conserve water, while increasing the grain yield and quality of drip-irrigated rice.

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Post-Anthesis Water-stressed Barley Maintains Grain Specific Weight Through Altered Grain Composition and Plant Architecture.

Publications

Post-Anthesis Water-stressed Barley Maintains Grain Specific Weight Through Altered Grain Composition and Plant Architecture.

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