Unknown

Dataset Information

0

Human Endometrial Carcinogenesis Is Associated with Significant Reduction in Long Non-Coding RNA, TERRA.


ABSTRACT: Telomeres are transcribed as long non-coding RNAs called TERRAs (Telomeric repeat containing RNA) that participate in a variety of cellular regulatory functions. High telomerase activity (TA) is associated with endometrial cancer (EC). This study aimed to examine the levels of three TERRAs, transcribed at chromosomes 1q-2q-4q-10q-13q-22q, 16p and 20q in healthy (n = 23) and pathological (n = 24) human endometrium and to examine their association with cellular proliferation, TA and telomere lengths. EC samples demonstrated significantly reduced levels of TERRAs for Chromosome 16p (Ch-16p) (p < 0.002) and Chromosome 20q (Ch-20q) (p = 0.0006), when compared with the postmenopausal samples. No significant correlation was found between TERRA levels and TA but both Ch-16p and Ch-20q TERRA levels negatively correlated with the proliferative marker Ki67 (r = -0.35, p = 0.03 and r = -0.42, p = 0.01 respectively). Evaluation of single telomere length analysis (STELA) at XpYp telomeres demonstrated a significant shortening in EC samples when compared with healthy tissues (p = 0.002). We detected TERRAs in healthy human endometrium and observed altered individual TERRA-specific levels in malignant endometrium. The negative correlation of TERRAs with cellular proliferation along with their significant reduction in EC may suggest a role for TERRAs in carcinogenesis and thus future research should explore TERRAs as potential therapeutic targets in EC.

SUBMITTER: Adishesh M 

PROVIDER: S-EPMC7698627 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Human Endometrial Carcinogenesis Is Associated with Significant Reduction in Long Non-Coding RNA, TERRA.

Adishesh Meera M   Alnafakh Rafah R   Baird Duncan M DM   Jones Rhiannon E RE   Simon Shannon S   Button Lucy L   Kamal Areege M AM   Kirwan John J   DeCruze S Bridget SB   Drury Josephine J   Saretzki Gabriele G   Hapangama Dharani K DK  

International journal of molecular sciences 20201118 22


Telomeres are transcribed as long non-coding RNAs called TERRAs (Telomeric repeat containing RNA) that participate in a variety of cellular regulatory functions. High telomerase activity (TA) is associated with endometrial cancer (EC). This study aimed to examine the levels of three TERRAs, transcribed at chromosomes 1q-2q-4q-10q-13q-22q, 16p and 20q in healthy (<i>n</i> = 23) and pathological (<i>n</i> = 24) human endometrium and to examine their association with cellular proliferation, TA and  ...[more]

Similar Datasets

| S-EPMC4467107 | biostudies-literature
| S-EPMC9962265 | biostudies-literature
| S-EPMC4924730 | biostudies-literature
| S-EPMC6361746 | biostudies-literature
| S-EPMC6551655 | biostudies-literature
| S-EPMC7471146 | biostudies-literature
| S-EPMC9584683 | biostudies-literature
| S-EPMC4898434 | biostudies-literature
| 2693518 | ecrin-mdr-crc
| S-EPMC2943627 | biostudies-literature