Unknown

Dataset Information

0

Detection of viral RNA fragments in human iPSC cardiomyocytes following treatment with extracellular vesicles from SARS-CoV-2 coding sequence overexpressing lung epithelial cells.


ABSTRACT: Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global pandemic. The prevalence/severity of COVID-19 is higher among patients with cardiovascular risk factors. Despite the expression of angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV-2 infection, in cardiomyocytes, there has been no conclusive evidence of direct viral infection although the presence of viral genome within COVID-19 patients' hearts has been reported. Here, we overexpressed SARS-CoV-2 genes in A549 lung epithelial cells. We then isolated extracellular vesicles (EVs) and detected the presence of viral RNA within these EVs. We observed that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are receptive to these EVs, and viral genes were detectable in the cardiomyocytes. Accordingly, the uptake of viral RNA-harboring EVs led to an upregulation of inflammation-related genes in hiPSC-CMs. Thus, our findings indicate that SARS-CoV-2 RNA containing EVs represents an indirect route of viral RNA entry into cardiomyocytes.

SUBMITTER: Kwon Y 

PROVIDER: S-EPMC7703503 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

2024-10-02 | E-MTAB-13966 | biostudies-arrayexpress
| S-EPMC10334823 | biostudies-literature
| S-EPMC7652393 | biostudies-literature
| S-EPMC5912190 | biostudies-literature
| S-EPMC6209632 | biostudies-literature
| S-EPMC9684507 | biostudies-literature
| S-EPMC8870779 | biostudies-literature
2024-10-03 | GSE275104 | GEO
| S-EPMC5678888 | biostudies-literature
| S-EPMC6302004 | biostudies-literature