Passive bilateral leg cycling with concomitant regional circulatory occlusion for testing mechanoreflex-metaboreflex interactions in humans.

ABSTRACT: PURPOSE:The exercise pressor reflex (EPR) plays a fundamental role in physiological reactions to exercise in humans and in the pathophysiology of cardiovascular disorders. There is no "gold standard" method for EPR assessment; therefore, we propose a new protocol for testing interactions between the muscle mechanoreflex and metaboreflex (major components of EPR). METHODS:Thirty-four healthy subjects (mean age [± standard deviation] 24?±?4 years, 22 men) were enrolled in the study. During the study, the hemodynamic and ventilatory parameters of these subjects were continuously monitored using our proposed assessment method. This assessment method consists of an initial 5-min rest period (baseline) followed by 5 min of passive cycling (PC) on an automated cycle ergometer (mechanoreceptor stimulation), after which tourniquet cuffs located bilaterally on the upper thighs are inflated for 3 min to evoke venous and arterial regional circulatory occlusion (CO) during PC (metaboreceptor stimulation). Deflation of the tourniquet cuffs is followed by a second 5 min of PC and finally by a 5-min recovery time. The control test comprises a 5-min rest period, followed by 3 min of CO only and a final 5-min recovery. RESULTS:Mean arterial pressure (MAP) and minute ventilation (MV) increased significantly during PC (MAP: from 90?±?9.3 to 95?±?9.7 mmHg; MV: from 11.5?±?2.5 to 13.5?±?2.9 L/min; both p?

SUBMITTER: Lis A 

PROVIDER: S-EPMC7704518 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature