A cyanobacterial photorespiratory bypass model to enhance photosynthesis by rerouting photorespiratory pathway in C₃ plants.

ABSTRACT: Plants employ photosynthesis to produce sugars for supporting their growth. During photosynthesis, an enzyme Ribulose 1,5 bisphosphate carboxylase/oxygenase (Rubisco) combines its substrate Ribulose 1,5 bisphosphate (RuBP) with CO₂ to produce phosphoglycerate (PGA). Alongside, Rubisco also takes up O₂ and produce 2-phosphoglycolate (2-PG), a toxic compound broken down into PGA through photorespiration. Photorespiration is not only a resource-demanding process but also results in CO₂ loss which affects photosynthetic efficiency in C₃ plants. Here, we propose to circumvent photorespiration by adopting the cyanobacterial glycolate decarboxylation pathway into C₃ plants. For that, we have integrated the cyanobacterial glycolate decarboxylation pathway into a kinetic model of C₃ photosynthetic pathway to evaluate its impact on photosynthesis and photorespiration. Our results show that the cyanobacterial glycolate decarboxylation bypass model exhibits a 10% increase in net photosynthetic rate (A) in comparison with C₃ model. Moreover, an increased supply of intercellular CO₂ (C_i) from the bypass resulted in a 54.8% increase in PGA while reducing photorespiratory intermediates including glycolate (-?49%) and serine (-?32%). The bypass model, at default conditions, also elucidated a decline in phosphate-based metabolites including RuBP (-?61.3%). The C₃ model at elevated level of inorganic phosphate (Pi), exhibited a significant change in RuBP (+?355%) and PGA (-?98%) which is attributable to the low availability of C_i. Whereas, at elevated Pi, the bypass model exhibited an increase of 73.1% and 33.9% in PGA and RuBP, respectively. Therefore, we deduce a synergistic effect of elevation in CO₂ and Pi pool on photosynthesis. We also evaluated the integrative action of CO₂, Pi, and Rubisco carboxylation activity (V_cmax) on A and observed that their simultaneous increase raised A by 26%, in the bypass model. Taken together, the study potentiates engineering of cyanobacterial decarboxylation pathway in C₃ plants to bypass photorespiration thereby increasing the overall efficiency of photosynthesis.

SUBMITTER: Khurshid G 

PROVIDER: S-EPMC7705653 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature