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ELISA detection of SARS-CoV-2 antibodies in saliva.

ABSTRACT: To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva samples, achieving 84.2% sensitivity and 100% specificity in a set of 149 clinical samples. This strategy will enable widespread, affordable testing for patients who experienced this disease, whilst minimizing exposure risk for healthcare workers.

SUBMITTER: MacMullan MA

PROVIDER: S-EPMC7705674 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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ELISA detection of SARS-CoV-2 antibodies in saliva.

MacMullan Melanie A MA Ibrayeva Albina A Trettner Kylie K Deming Laura L Das Sudipta S Tran Frances F Moreno Jose Ricardo JR Casian Joseph G JG Chellamuthu Prithivi P Kraft Jeffrey J Kozak Kenneth K Turner Fred E FE Slepnev Vladimir I VI Le Page Lydia M LM

Scientific reports 20201130 1

To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva samples, achieving 84.2% sensitivity and 100% specificity in a set of 149 clinical samples. This strategy will enable widespread, affordable testing for patients who experienced this disease, whilst minimiz ...[more]

PMID: 33257702

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