Ontology highlight
ABSTRACT:
SUBMITTER: Shimono H
PROVIDER: S-EPMC7705701 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Shimono Hiroaki H Kaida Atsushi A Homma Hisao H Nojima Hitomi H Onozato Yusuke Y Harada Hiroyuki H Miura Masahiko M
Scientific reports 20201130 1
In this study, we examined the fluctuation in radioresponse of HeLa cells during the cell cycle. For this purpose, we used HeLa cells expressing two types of fluorescent ubiquitination-based cell cycle indicators (Fucci), HeLa-Fucci (CA)2 and HeLa-Fucci (SA), and combined this approach with the micronucleus (MN) assay to assess radioresponse. The Fucci system distinguishes cell cycle phases based on the colour of fluorescence and cell morphology under live conditions. Time-lapse imaging allowed ...[more]