Dataset Information

Evaluating the Utility of Carbon Isotope Discrimination for Wheat Breeding in the Pacific Northwest.

ABSTRACT: Many wheat (Triticum aestivum L.) production regions are threatened annually by drought stress. Carbon isotope discrimination (?) has been identified as a potentially useful trait in breeding for improved drought tolerance in certain environments. Broad use of ? as a selection criterion is limited, however, mainly due to an inconsistent relationship observed between grain yield and ? and, to a lesser extent, because of the high resource demand associated with phenotyping. The efficiency of selection may be improved by the identification and verification of molecular markers for use in marker-assisted selection (MAS), and a reliable relationship to grain yield may be established based on a location's total amount and distribution of precipitation over the growing season. Given the environmental variability in precipitation dynamics, it is necessary to evaluate this relationship in target breeding environments. In this study, grain ? was collected on a panel of 480 advanced soft white winter wheat varieties grown in five Pacific Northwest environments. A genome-wide association study approach was used to evaluate the amenability of grain ? to MAS. The genetic architecture of grain ? was determined to be characterized by multiple, small effect marker-trait associations with limited repeatability across environments, suggesting that MAS will be ineffective at improving ? selection efficiency. Further, the relationship between grain yield and ? ranged from neutral (r = -0.01) to moderately positive (r = 0.44) in the target environments. Such moderate correlations, coupled with variability in this relationship, indicate that direct selection for ? may not be beneficial.

SUBMITTER: Dixon LS

PROVIDER: S-EPMC7706333 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Evaluating the Utility of Carbon Isotope Discrimination for Wheat Breeding in the Pacific Northwest.

Dixon Liam S LS Godoy Jayfred V JV Carter Arron H AH

Plant phenomics (Washington, D.C.) 20190829

Many wheat (Triticum aestivum L.) production regions are threatened annually by drought stress. Carbon isotope discrimination (Δ) has been identified as a potentially useful trait in breeding for improved drought tolerance in certain environments. Broad use of Δ as a selection criterion is limited, however, mainly due to an inconsistent relationship observed between grain yield and Δ and, to a lesser extent, because of the high resource demand associated with phenotyp ...[more]

PMID: 33313527

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Project description:Eyespot, caused by the soil-borne necrotrophic fungi Oculimacula yallundae and O. acuformis, is a disease of major economic significance for wheat, barley and rye. Pacific Northwest (PNW) winter wheat (Triticum aestivum L.) grown in areas of high rainfall and moderate winters is most vulnerable to infection. The objective of this research was to identify novel genomic regions associated with eyespot resistance in winter wheat adapted to the PNW. Two winter wheat panels of 469 and 399 lines were compiled for one of the first genome-wide association studies (GWAS) of eyespot resistance in US winter wheat germplasm. These panels were genotyped with the Infinium 9K and 90K iSelect SNP arrays. Both panels were phenotyped for disease resistance in a two-year field study and in replicated growth chamber trials. Growth chamber trials were used to evaluate the genetic resistance of O. acuformis and O. yallundae species separately. Best linear unbiased predictors (BLUPs) were calculated across all field and growth chamber environments. A total of 73 marker-trait associations (MTAs) were detected on nine different chromosomes (1A, 2A, 2B, 4A, 5A, 5B, 7A, 7B and 7D) that were significantly associated (p-value <0.001) with eyespot resistance in Panel A, and 19 MTAs on nine different chromosomes (1A, 1B, 2A, 2D, 3B, 5A, 5B, 7A, and 7B) in Panel B. The most significant SNPs were associated with Pch1 and Pch2 resistance genes on the long arms of chromosome 7D and 7A. Most of the novel MTAs appeared to have a minor effect on reducing eyespot disease. Nevertheless, eyespot disease scores decreased as the number of resistance alleles increased. Seven SNP markers, significantly associated with reducing eyespot disease across environments and in the absence and presence of Pch1 were identified. These markers were located on chromosomes 2A (IWB8331), 5A (IWB73709), 5B (IWB47298), 7AS (IWB47160), 7B (IWB45005) and two SNPs (Ex_c44379_2509 and IAAV4340) had unknown map positions. The additive effect of the MTAs explained most of the remaining phenotypic variation not accounted for by Pch1 or Pch2. This study provides breeders with adapted germplasm and novel sources of eyespot resistance to be used in the development of superior cultivars with increased eyespot resistance.

Project description:Microbial transformations of organic carbon (OC) generate a large flux of CO2 into the atmosphere and influence the C balance of terrestrial and aquatic ecosystems. Yet, inherent heterogeneity in natural environments precludes direct quantification of multiple microbial C fluxes that underlie CO2 production. Here we used a continuous flow bioreactor coupled with a stable C isotope analyzer to determine the effects of temperature and C availability (cellobiose concentration) on C fluxes and 13C discrimination of a microbial population growing at steady-state in a homogeneous, well-mixed environment. We estimated C uptake affinity and C use efficiency (CUE) to characterize the physiological responses of microbes to changing environmental conditions. Temperature increased biomass-C specific respiration rate and C uptake affinity at lower C availability, but did not influence those parameters at higher C availability. CUE decreased non-linearly with increasing temperature. The non-linear, negative relationship between CUE and temperature was more pronounced under lower C availability than under relatively high C availability. We observed stable isotope fractionation between C substrate and microbial biomass C (7~12‰ depletion), and between microbial biomass and respired CO2 (4~10‰ depletion). Microbial discrimination against 13C-containing cellobiose during C uptake was influenced by temperature and C availability, while discrimination during respiration was only influenced by C availability. Shifts in C uptake affinity with temperature and C availability may have modified uptake-induced 13C fractionation. By stressing the importance of C availability on temperature responses of microbial C fluxes, C uptake affinity, CUE, and isotopic fractionation, this study contributes to a fundamental understanding of C flow through microbes. This will help guide parameterization of microbial responses to varying temperature and C availability within Earth-system models.

Dataset Information

Evaluating the Utility of Carbon Isotope Discrimination for Wheat Breeding in the Pacific Northwest.

Publications

Evaluating the Utility of Carbon Isotope Discrimination for Wheat Breeding in the Pacific Northwest.

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