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The complete chloroplast genome of Scutellaria indica var. coccinea (Lamiaceae), an endemic taxon in Korea.

ABSTRACT: Scutellaria indica var. coccinea (Lamiaceae) is an endemic taxon in Jeju Island, Korea. We present the complete chloroplast genome sequence of S. indica var. coccinea. Its genome size is 151,956?bp in length comprising a large single copy of 83,951?bp, a small single copy of 17,539?bp, and a pair of inverted repeats measuring 25,233?bp. The complete genome contains 115 genes including 80 protein-coding genes, four rRNAs, and 31 tRNAs. Phylogenetic analysis of this species with 10 published chloroplast genomes in Lamiaceae showed that S. insignis is a sister to S. indica var. coccinea.

SUBMITTER: Lee Y

PROVIDER: S-EPMC7706630 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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The complete chloroplast genome of Scutellaria indica var. coccinea (Lamiaceae), an endemic taxon in Korea.

Lee Yoonkyung Y Kim Sangtae S

Mitochondrial DNA. Part B, Resources 20190713 2

Scutellaria indica var. coccinea (Lamiaceae) is an endemic taxon in Jeju Island, Korea. We present the complete chloroplast genome sequence of S. indica var. coccinea. Its genome size is 151,956 bp in length comprising a large single copy of 83,951 bp, a small single copy of 17,539 bp, and a pair of inverted repeats measuring 25,233 bp. The complete genome contains 115 genes including 80 protein-coding genes, four rRNAs, and 31 tRNAs. Phylogenetic analysis of this spe ...[more]

PMID: 33365616

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Project description:Terpenoids are the largest class of natural products with complex structures and extensive bioactivities; their scaffolds are generated by diverse terpenoid synthases (TPSs) from a limited number of isoprenoid diphosphate precursors. Promiscuous TPSs play important roles in the evolution of terpenoid chemodiversity, but they remain largely unappreciated. Here, an extremely promiscuous terpenoid synthase (CcTPS1) of the TPS-b subfamily was cloned and functionally characterized from a leaf-specific transcriptome of the Lamiaceae plant Colquhounia coccinea var. mollis. CcTPS1 is the first sester-/di-/sesqui-/mono-TPS identified from the plant kingdom, accepting C25/C20/C15/C10 diphosphate substrates to generate a panel of sester-/di-/sesqui-/mono-terpenoids. Engineered Escherichia coli expressing CcTPS1 produced three previously unreported terpenoids (two sesterterpenoids and a diterpenoid) with rare cyclohexane-containing skeletons, along with four sesquiterpenoids and one monoterpenoid. Their structures were elucidated by extensive nuclear magnetic resonance spectroscopy. Nicotiana benthamiana transiently expressing CcTPS1 also produced the diterpenoid and sesquiterpenoids, demonstrating the enzyme’s promiscuity in planta. Its highly leaf-specific expression pattern combined with detectable terpenoid products in leaves of C. coccinea var. mollis and N. benthamiana expressing CcTPS1 suggested that CcTPS1 was mainly responsible for diterpenoid and sesquiterpenoid biosynthesis in plants. CcTPS1 expression and the terpenoid products could be induced by methyl jasmonate, suggesting their possible role in plant–environment interaction. CcTPS1 was localized to the cytosol and may differ from mono-TPSs in subcellular compartmentalization and substrate tolerance. These findings will greatly aid our understanding of plant TPS evolution and terpenoid chemodiversity; they also highlight the enormous potential of transcriptome mining and heterologous expression for the exploration of unique enzymes and natural products hidden in plants. The astounding chemodiversity of terpenoids is mainly ascribed to diverse terpenoid synthases. This study reports the characterization of an extremely promiscuous terpenoid synthase from the Lamiaceae plant Colquhounia coccinea var. mollis. It accepts C25/C20/C15/C10 substrates to generate a panel of sester-/di-/sesqui-/mono-terpenoids, including three previously undescribed terpenoids: two sesterterpenoids and a diterpenoid.

Dataset Information

The complete chloroplast genome of Scutellaria indica var. coccinea (Lamiaceae), an endemic taxon in Korea.

Publications

The complete chloroplast genome of <i>Scutellaria indica</i> var. <i>coccinea</i> (Lamiaceae), an endemic taxon in Korea.

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