Dataset Information

The complete mitochondrial genome of the Antarctic marine triclad, Obrimoposthia Wandeli (Platyhelminthes, Tricladida, Maricola).

ABSTRACT: For the first time, we report the complete mitochondrial genome (mitogenome) sequence of the marine triclad species, Obrimoposthia wandeli. The complete mitogenome of O. wandeli was 15,185?bp in length, contains 12 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs). Compared to previously reported gene orders from the order Tricladida, O. wandeli had unique gene order. We constructed a phylogenetic tree based on the mitogenomes belonging to Rhabdocoela, Polycladida, and Tricladida and confirmed that O. wandeli is located in the basal position in the Tricladida.

SUBMITTER: Yang HM

PROVIDER: S-EPMC7706869 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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The complete mitochondrial genome of the Antarctic marine triclad, Obrimoposthia Wandeli (Platyhelminthes, Tricladida, Maricola).

Yang Hee-Min HM Ji Su-Jung SJ Min Gi-Sik GS

Mitochondrial DNA. Part B, Resources 20190713 2

For the first time, we report the complete mitochondrial genome (mitogenome) sequence of the marine triclad species, Obrimoposthia wandeli. The complete mitogenome of O. wandeli was 15,185 bp in length, contains 12 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs). Compared to previously reported gene orders from the order Tricladida, O. wandeli had unique gene order. We constructed a phylogenetic tree based on the mitogenomes belonging to R ...[more]

PMID: 33365606

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Project description:BackgroundDugesia sicula is the only species of its genus not presenting an endemic or restricted distribution within the Mediterranean area. It mostly comprises fissiparous populations (asexual reproduction by body division and regeneration), most likely sexually sterile, and characterized by an extremely low genetic diversity interpreted as the consequence of a recent anthropic expansion. However, its fissiparous reproduction can result in an apparent lack of diversity within the species, since genetic variation within individuals can be as large as between them because most individuals within a population are clones. We have estimated haplotype and nucleotide diversity of cytochrome oxidase I within and among individuals along the species distribution of a broad sample of D. sicula, including asexual and the two only sexual populations known today; and predicted its potential distribution based on climatic variables. Our aim was to determine the centre of colonisation origin, whether the populations are recent, and whether the species is expanding.ResultsThe species presents 3 most frequent haplotypes, differing in a maximum of 11 base pairs. As expected from their fissiparous mode of reproduction, in half of all the analysed localities many individuals have multiple heteroplasmic haplotypes. The distribution of haplotypes is not geographically structured; however, the distribution of haplotypes and heteroplasmic populations shows higher diversity in the central Mediterranean region. The potential distribution predicted by climatic variables based modelling shows a preference for coastal areas and fits well with the observed data.ConclusionsThe distribution and frequency of the most frequent haplotypes and the presence of heteroplasmic individuals allow us to gain an understanding of the recent history of the species, together with previous knowledge on its phylogenetic relationships and age: The species most probably originated in Africa and dispersed through the central Mediterranean. After one or multiple populations became triploid and fissiparous, the species colonized the Mediterranean basin, likely both by its own means and helped by human activities. Its present distribution practically fulfils its potential distribution as modelled with climatic variables. Its prevalence in coastal regions with higher water temperatures predicts a likely future expansion to northern and more interior areas following the increase in temperatures due to climate change.

Project description:BackgroundMitogenomics data, i.e. complete mitochondrial genome sequences, are popular molecular markers used for phylogenetic, phylogeographic and ecological studies in different animal lineages. Their comparative analysis has been used to shed light on the evolutionary history of given taxa and on the molecular processes that regulate the evolution of the mitochondrial genome. A considerable literature is available in the fields of invertebrate biochemical and ecophysiological adaptation to extreme environmental conditions, exemplified by those of the Antarctic. Nevertheless, limited molecular data are available from terrestrial Antarctic species, and this study represents the first attempt towards the description of a mitochondrial genome from one of the most widespread and common collembolan species of Antarctica.ResultsIn this study we describe the mitochondrial genome of the Antarctic collembolan Cryptopygus antarcticus Willem, 1901. The genome contains the standard set of 37 genes usually present in animal mtDNAs and a large non-coding fragment putatively corresponding to the region (A+T-rich) responsible for the control of replication and transcription. All genes are arranged in the gene order typical of Pancrustacea. Three additional short non-coding regions are present at gene junctions. Two of these are located in positions of abrupt shift of the coding polarity of genes oriented on opposite strands suggesting a role in the attenuation of the polycistronic mRNA transcription(s). In addition, remnants of an additional copy of trnL(uag) are present between trnS(uga) and nad1. Nucleotide composition is biased towards a high A% and T% (A+T = 70.9%), as typically found in hexapod mtDNAs. There is also a significant strand asymmetry, with the J-strand being more abundant in A and C. Within the A+T-rich region, some short sequence fragments appear to be similar (in position and primary sequence) to those involved in the origin of the N-strand replication of the Drosophila mtDNA.ConclusionThe mitochondrial genome of C. antarcticus shares several features with other pancrustacean genomes, although the presence of unusual non-coding regions is also suggestive of molecular rearrangements that probably occurred before the differentiation of major collembolan families. Closer examination of gene boundaries also confirms previous observations on the presence of unusual start and stop codons, and suggests a role for tRNA secondary structures as potential cleavage signals involved in the maturation of the primary transcript. Sequences potentially involved in the regulation of replication/transcription are present both in the A+T-rich region and in other areas of the genome. Their position is similar to that observed in a limited number of insect species, suggesting unique replication/transcription mechanisms for basal and derived hexapod lineages. This initial description and characterization of the mitochondrial genome of C. antarcticus will constitute the essential foundation prerequisite for investigations of the evolutionary history of one of the most speciose collembolan genera present in Antarctica and other localities of the Southern Hemisphere.

Dataset Information

The complete mitochondrial genome of the Antarctic marine triclad, Obrimoposthia Wandeli (Platyhelminthes, Tricladida, Maricola).

Publications

The complete mitochondrial genome of the Antarctic marine triclad, <i>Obrimoposthia Wandeli</i> (Platyhelminthes, Tricladida, Maricola).

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