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The complete chloroplast genome of Eichhornia crassipes (Pontederiaceae) and phylogeny of commelinids.

ABSTRACT: Eichhornia crassipes is a floating aquatic plant native to the American tropics. Here we reported and characterized the first complete chloroplast (cp) genome of Eichhornia crassipes and analyzed the phylogenomic relationship of Commelinids based on complete chloroplast sequences. The complete chloroplast genome of Eichhornia crassipes has a typical quadripartite structure with a total length of 161,783?bp. A total of 124 genes, including 85 encoding genes, 38 transfer RNA genes, and 8 ribosomal RNA genes were annotated. The phylogenomic study validated the phylogenetic position of Eichhornia crassipes and showed that four species from Commelinales form a monophyletic group sister to Zingiberales.

SUBMITTER: Ma Q

PROVIDER: S-EPMC7706893 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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The complete chloroplast genome of Eichhornia crassipes (Pontederiaceae) and phylogeny of commelinids.

Ma Qing Q Lu Yin Y

Mitochondrial DNA. Part B, Resources 20190923 2

Eichhornia crassipes is a floating aquatic plant native to the American tropics. Here we reported and characterized the first complete chloroplast (cp) genome of Eichhornia crassipes and analyzed the phylogenomic relationship of Commelinids based on complete chloroplast sequences. The complete chloroplast genome of Eichhornia crassipes has a typical quadripartite structure with a total length of 161,783 bp. A total of 124 genes, including 85 encoding genes, 38 transfer RNA g ...[more]

PMID: 33365911

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Project description:Morchella crassipes (Vent.) Pers., a typical yellow morel species with high economic value, is mainly distributed in the low altitude plains of Eurasia. However, rare research has been performed on its genomics and polarity, thus limiting its research and development. Here, we reported a fine physical map of the nuclear genome at the subchromosomal-scale and the complete mitochondrial genome of M. crassipes. The complete size of the nuclear genome was 56.7 Mb, and 23 scaffolds were assembled, with eight of them being complete chromosomes. A total of 11,565 encoding proteins were predicted. The divergence time analysis showed that M. crassipes representing yellow morels differentiated with black morels at ~33.98 Mya (million years), with 150 gene families contracted and expanded in M. crassipes versus the two black morels (M. snyderi and M. importuna). Furthermore, 409 CAZYme genes were annotated in M. crassipes, containing almost all plant cell wall degrading enzymes compared with the mycorrhizal fungi (truffles). Genomic annotation of mating type loci and amplification of the mating genes in the monospore population was conducted, the results indicated that M. crassipes is a heterothallic fungus. Additionally, a complete circular mitochondrial genome of M. crassipes was assembled, the size reached as large as 531,195 bp. It can be observed that the strikingly large size was the biggest up till now, coupled with 14 core conserved mitochondrial protein-coding genes, two rRNAs, 31 tRNAs, 51 introns, and 412 ncORFs. The total length of intron sequences accounted for 53.67% of the mitochondrial genome, with 19 introns having a length over 5 kb. Particularly, 221 of 412 ncORFs were distributed within 51 introns, and the total length of the ncORFs sequence accounted for 40.83% of the mitochondrial genome, and 297 ncORFs had expression activity in the mycelium stage, suggesting their potential functions in M. crassipes. Meanwhile, there was a high degree of repetition (51.31%) in the mitochondria of M. crassipes. Thus, the large number of introns, ncORFs and internal repeat sequences may contribute jointly to the largest fungal mitochondrial genome to date. The fine physical maps of nuclear genome and mitochondrial genome obtained in this study will open a new door for better understanding of the mysterious species of M. crassipes.

Project description:IntroductionThe endosphere of a plant is an interface containing a thriving community of endobacteria that can affect plant growth and potential for bioremediation. Eichhornia crassipes is an aquatic macrophyte, adapted to estuarine and freshwater ecosystems, which harbors a diverse bacterial community. Despite this, we currently lack a predictive understanding of how E. crassipes taxonomically structure the endobacterial community assemblies across distinct habitats (root, stem, and leaf).MethodsIn the present study, we assessed the endophytic bacteriome from different compartments using 16S rRNA gene sequencing analysis and verified the in vitro plant beneficial potential of isolated bacterial endophytes of E. crassipes.Results and discussionPlant compartments displayed a significant impact on the endobacterial community structures. Stem and leaf tissues were more selective, and the community exhibited a lower richness and diversity than root tissue. The taxonomic analysis of operational taxonomic units (OTUs) showed that the major phyla belonged to Proteobacteria and Actinobacteriota (> 80% in total). The most abundant genera in the sampled endosphere was Delftia in both stem and leaf samples. Members of the family Rhizobiaceae, such as in both stem and leaf samples. Members of the family Rhizobiaceae, such as Allorhizobium- Neorhizobium-Pararhizobium-Rhizobium were mainly associated with leaf tissue, whereas the genera Nannocystis and Nitrospira from the families Nannocystaceae and Nitrospiraceae, respectively, were statistically significantly associated with root tissue. Piscinibacter and Steroidobacter were putative keystone taxa of stem tissue. Most of the endophytic bacteria isolated from E. crassipes showed in vitro plant beneficial effects known to stimulate plant growth and induce plant resistance to stresses. This study provides new insights into the distribution and interaction of endobacteria across different compartments of E. crassipes Future study of endobacterial communities, using both culture-dependent and -independent techniques, will explore the mechanisms underlying the wide-spread adaptability of E. crassipesto various ecosystems and contribute to the development of efficient bacterial consortia for bioremediation and plant growth promotion.

Dataset Information

The complete chloroplast genome of Eichhornia crassipes (Pontederiaceae) and phylogeny of commelinids.

Publications

The complete chloroplast genome of <i>Eichhornia crassipes</i> (Pontederiaceae) and phylogeny of commelinids.

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