Dataset Information
Identifying Key MicroRNAs Targeted by Narenmandula in a Rodent Nephropathy Model.
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ABSTRACT:
Methods: Fifteen rats exhibiting normal renal function were randomized to three study arms. Nephropathy was induced in n?=?10 rats using doxorubicin hydrochloride, followed by either Narenmandula treatment (treatment group) or no treatment (control group). In n?=?5 rats, no doxorubicin was given and renal function remained unchanged (healthy group). Microarray analysis identified miRNAs which were differentially expressed (DE-miRNAs) between groups. Target genes of DE-miRNAs were predicted using miRWalk version 2.0, followed by enrichment analysis using DAVID, and construction of the miRNA coregulatory network using Cytoscape.
Results: Nephropathy was successfully induced, with doxorubicin resulting in differential expression of 3645 miRNAs (1324 upregulated and 2321 downregulated). Narenmandula treatment induced differential expression of a total of 159 miRNAs (102 upregulated and 57 downregulated). Upregulated DE-miRNAs (e.g., miR-497-5p, miR-195-5p, miR-181a-5p, miR-181c-5p, and miR-30e-5p) and downregulated DE-miRNAs (e.g., miR-330-3p and miR-214-3p) regulated a high number of target genes. Moreover, the miRNA pairs (e.g., miR-195-5p-miR-497-5p, miR-181a-5p-miR-181c-5p, and miR-30e-5p-miR-30a-5p) coregulated a high number of genes. Enrichment analysis indicated functional synergy between miR-30e-5p-miR-30a-3p, miR-34a-5p-miR-30e-5p, miR-30e-5p-miR-195-3p, and miR-30a-3p-miR-195-3p pairs.
Conclusion: Narenmandula may modulate doxorubicin-induced nephropathy via targeting miR-497-5p, miR-195-5p, miR-181a-5p, miR-181c-5p, miR-30e-5p, miR-330-3p, miR-214-3p, miR-34a-5p, miR-30a-3p, and miR-30a-5p.
SUBMITTER: Wang X
PROVIDER: S-EPMC7707998 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
Identifying Key MicroRNAs Targeted by Narenmandula in a Rodent Nephropathy Model.
Evidence-based complementary and alternative medicine : eCAM 20201124
<h4>Background</h4>Untreated nephropathy can progress to renal failure. The traditional Mongolian remedy Narenmandula regulates the kidney "yang." This study aimed to identify key microRNAs (miRNAs) targeted by Narenmandula in a rat model of nephropathy.<h4>Methods</h4>Fifteen rats exhibiting normal renal function were randomized to three study arms. Nephropathy was induced in <i>n</i> = 10 rats using doxorubicin hydrochloride, followed by either Narenmandula treatment (treatment group) or no tr ...[more]