Ontology highlight
ABSTRACT:
SUBMITTER: Jung WH
PROVIDER: S-EPMC7708044 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Jung Wei-Hung WH Chen Enze E Veneziano Remi R Gaitanaros Stavros S Chen Yun Y
Nucleic acids research 20201201 21
The axial stiffness of DNA origami is determined as a function of key nanostructural characteristics. Different constructs of two-helix nanobeams with specified densities of nicks and Holliday junctions are synthesized and stretched by fluid flow. Implementing single particle tracking to extract force-displacement curves enables the measurement of DNA origami stiffness values at the enthalpic elasticity regime, i.e. for forces larger than 15 pN. Comparisons between ligated and nicked helices sho ...[more]