Design and application of circular RNAs with protein-sponge function.
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ABSTRACT:
SUBMITTER: Schreiner S
PROVIDER: S-EPMC7708053 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Design and application of circular RNAs with protein-sponge function.
Schreiner Silke S Didio Anna A Hung Lee-Hsueh LH Bindereif Albrecht A
Nucleic acids research 20201201 21
Circular RNAs (circRNAs) are a class of noncoding RNAs, generated from pre-mRNAs by circular splicing of exons and functionally largely uncharacterized. Here we report on the design, expression, and characterization of artificial circRNAs that act as protein sponges, specifically binding and functionally inactivating hnRNP (heterogeneous nuclear ribonucleoprotein) L. HnRNP L regulates alternative splicing, depending on short CA-rich RNA elements. We demonstrate that designer hnRNP L-sponge circR ...[more]
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