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Rational design of minimal synthetic promoters for plants.

ABSTRACT: Promoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors. Previously, a paucity of data for cis-regulatory elements in plants meant that it was challenging to determine which sequence elements in plant promoter sequences contributed to transcriptional function. In this study, we have identified functional elements in the promoters of plant genes and plant pathogens that utilize plant transcriptional machinery for gene expression. We have established a quantitative experimental system to investigate transcriptional function, investigating how identity, density and position contribute to regulatory function. We then identified permissive architectures for minimal synthetic plant promoters enabling the computational design of a suite of synthetic promoters of different strengths. These have been used to regulate the relative expression of output genes in simple genetic devices.

SUBMITTER: Cai YM

PROVIDER: S-EPMC7708054 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Rational design of minimal synthetic promoters for plants.

Cai Yao-Min YM Kallam Kalyani K Tidd Henry H Gendarini Giovanni G Salzman Amanda A Patron Nicola J NJ

Nucleic acids research 20201201 21

Promoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors. Previously, a paucity of data for cis-regulatory elements in plants meant that it was challenging to determine which sequence elements in plant promoter sequences contributed to transcriptional function. In this study, we have identified functional elements in the promoters of plant genes and plant pathogens that utilize plant transcriptional mac ...[more]

PMID: 32856047

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