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Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity.

ABSTRACT: Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.

SUBMITTER: Gorbachev DA

PROVIDER: S-EPMC7709005 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity.

Gorbachev Dmitry A DA Staroverov Dmitry B DB Lukyanov Konstantin A KA Sarkisyan Karen S KS

International journal of molecular sciences 20201120 22

Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxici ...[more]

PMID: 33233801

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