Transcriptome changes and polymyxin resistance of acid-adapted Escherichia coli O157:H7 ATCC 43889.

ABSTRACT:

Background

Acid treatment is commonly used for controlling or killing pathogenic microorganisms on medical devices and environments; however, inadequate acid treatment may cause acid tolerance response (ATR) and offer cross-protection against environmental stresses, including antimicrobials. This study aimed to characterise an Escherichia coli strain that can survive in the acidic gastrointestinal environment.

Results

We developed an acid-tolerant E. coli O157:H7 ATCC 43889 (ATCC 43889) strain that can survive at pH 2.75 via cell adaptation in low pH conditions. We also performed RNA sequencing and qRT-PCR to compare differentially expressed transcripts between acid-adapted and non-adapted cells. Genes related to stress resistance, including kdpA and bshA were upregulated in

SUBMITTER: Hwang D 

PROVIDER: S-EPMC7709258 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature