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Disposable silicon-based all-in-one micro-qPCR for rapid on-site detection of pathogens.


ABSTRACT: Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10?×?10?×?0.65?mm in size can be produced in 7?h, costing ~0.35 USD per device). We are able to quantitatively detect a 563?bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20?fg, equivalent to a single bacterium, at the 35th cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1?pg) with high specificity against SARS-CoV (2003).

SUBMITTER: Nunez-Bajo E 

PROVIDER: S-EPMC7710731 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Disposable silicon-based all-in-one micro-qPCR for rapid on-site detection of pathogens.

Nunez-Bajo Estefania E   Silva Pinto Collins Alexander A   Kasimatis Michael M   Cotur Yasin Y   Asfour Tarek T   Tanriverdi Ugur U   Grell Max M   Kaisti Matti M   Senesi Guglielmo G   Stevenson Karen K   Güder Firat F  

Nature communications 20201202 1


Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences  ...[more]

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