Ontology highlight
ABSTRACT:
SUBMITTER: Jia R
PROVIDER: S-EPMC7710758 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Jia Ruyu R Martens Chloe C Shekhar Mrinal M Pant Shashank S Pellowe Grant A GA Lau Andy M AM Findlay Heather E HE Harris Nicola J NJ Tajkhorshid Emad E Booth Paula J PJ Politis Argyris A
Nature communications 20201202 1
Proton-coupled transporters use transmembrane proton gradients to power active transport of nutrients inside the cell. High-resolution structures often fail to capture the coupling between proton and ligand binding, and conformational changes associated with transport. We combine HDX-MS with mutagenesis and MD simulations to dissect the molecular mechanism of the prototypical transporter XylE. We show that protonation of a conserved aspartate triggers conformational transition from outward-facin ...[more]